Szczegóły publikacji
Opis bibliograficzny
In vitro and in vivo studies of antibacterial coatings on titanium alloy implants for veterinary application / Magdalena ZIĄBKA, Katarzyna MATYSIAK, Katarzyna CHOLEWA-KOWALSKA, Agnieszka Kyzioł, Aleksandra Królicka, Rafał Sapierzyński, Monika Januchta-Kurmin, Igor Bissenik // International Journal of Molecular Sciences [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 1422-0067. — 2023 — vol. 24 iss. 9 art. no. 8114, s. 1–20. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 18–20, Abstr. — Publikacja dostępna online od: 2023-04-30
Autorzy (8)
- AGHZiąbka Magdalena
- AGHMatysiak Katarzyna Aleksandra
- AGHCholewa-Kowalska Katarzyna
- Kyzioł Agnieszka
- Królicka Aleksandra
- Sapierzyński Rafał
- Januchta-Kurmin Monika
- Bissenik Igor
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 146882 |
|---|---|
| Data dodania do BaDAP | 2023-05-30 |
| Tekst źródłowy | URL |
| DOI | 10.3390/ijms24098114 |
| Rok publikacji | 2023 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | International Journal of Molecular Sciences |
Abstract
The aim of this work was the evaluation of biological properties of hybrid coatings modified with Ag, Cu, and Zn nanoparticles (NPs) applied on TPLO medical implants by the sol-gel process. The implant coatings enriched with various concentrations of metallic NPs were investigated in the in vitro bactericidal efficacy tests against Gram+ and Gram- bacteria and pathogenic yeast. Next, the designed materials were tested on human osteosarcoma cell lines. The cells adhesion, proliferation, viability, and differentiation were investigated. The cell growth wasevaluated using SEM, and the metallic ion release was measured. The results revealed that the NPs concentration in the hybrid layers decreased with the incubation time. In the last stage, the implants were tested in vivo on six canine patients. Three months after the operation, the radiological evaluation of the performed anastomosis was carried out as well as the histopathological evaluation of tissue regeneration. The strongest bactericidal efficacy was observed for the layers containing AgNPs. Along with an increased concentration of metallic additives, a growing toxic effect was clearly observed. The most pronounced toxic effect was especially evident with the AgNPs concentration exceeding 1 mol %. In all the operated patients, no deviations were found during the follow-up examinations in the postoperative period. The low dose of AgNPs in the hybrid layer facilitated the tissue healing process. It was proven that silver nanoparticles may accelerate the bone healing process. The correct tissue reparation was observed.