Szczegóły publikacji
Opis bibliograficzny
In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery / Rafał Pokrowiecki, Tomasz Zaręba, Barbara SZARANIEC, Krzysztof Pałka, Agnieszka Mielczarek, Elżbieta Menaszek, Stefan Tyski // International Journal of Nanomedicine [Dokument elektroniczny]. - Czasopismo elektroniczne ; ISSN 1178-2013. — 2017 — vol. 12, s. 4285–4297. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 4296–4297, Abstr.
Autorzy (7)
- Pokrowiecki Rafał
- Zaręba Tomasz
- AGHSzaraniec Barbara
- Pałka Krzysztof
- Mielczarek Agnieszka
- Menaszek Elżbieta
- Tyski Stefan
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 106374 |
|---|---|
| Data dodania do BaDAP | 2017-08-22 |
| Tekst źródłowy | URL |
| DOI | 10.2147/IJN.S131163 |
| Rok publikacji | 2017 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | International Journal of Nanomedicine |
Abstract
The addition of an antibacterial agent to dental implants may provide the opportunity to decrease the percentage of implant failures due to peri-implantitis. For this purpose, in this study, the potential efficacy of nanosilver-doped titanium biomaterials was determined. Titanium disks were incorporated with silver nanoparticles over different time periods by Tollens reaction, which is considered to be an eco-friendly, cheap, and easy-to-perform method. The surface roughness, wettability, and silver release profile of each disc were measured. In addition, the antibacterial activity was also evaluated by using disk diffusion tests for bacteria frequently isolated from the peri-implant biofilm: Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus sanguis, Porphyromonas gingivalis, Staphylococcus aureus, and Escherichia coli. Cytotoxicity was evaluated in vitro in a natural human osteoblasts cell culture. The addition of nanosilver significantly increased the surface roughness and decreased the wettability in a dose-dependent manner. These surfaces were significantly toxic to all the tested bacteria following a 48-hour exposure, regardless of silver doping duration. A concentration of 0.05 ppm was sufficient to inhibit Gram-positive and Gram-negative species, with the latter being significantly more susceptible to silver ions. However, after the exposure of human osteoblasts to 0.1 ppm of silver ions, a significant decrease in cell viability was observed by using ToxiLight™ BioAssay Kit after 72 hours. Data from the present study indicated that the incorporation of nanosilver may influence the surface properties that are important in the implant healing process. The presence of nanosilver on the titanium provides an antibacterial activity related to the bacteria involved in peri-implantitis. Finally, the potential toxicological considerations of nanosilver should further be investigated, as both the antibacterial and cytotoxic properties may be observed at similar concentration ranges.