Szczegóły publikacji
Opis bibliograficzny
Safety assessment of the modified lactoperoxidase system – in vitro studies on human gingival fibroblasts / Marcin Magacz, Monika Papież, Dorota Kościelniak, Anna Jurczak, Karolina Kędziora, Elżbieta PAMUŁA, Wirginia Krzyściak // International Journal of Molecular Sciences [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 1422-0067. — 2023 — vol. 24 iss. 3 art. no. 2640, s. 1–13. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 12–13, Abstr. — Publikacja dostępna online od: 2023-01-30
Autorzy (7)
- Magacz Marcin
- Papież Monika
- Kościelniak Dorota
- Jurczak Anna
- Kędziora Karolina
- AGHPamuła Elżbieta
- Krzyściak Wirginia
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 145052 |
|---|---|
| Data dodania do BaDAP | 2023-02-01 |
| Tekst źródłowy | URL |
| DOI | 10.3390/ijms24032640 |
| Rok publikacji | 2023 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | International Journal of Molecular Sciences |
Abstract
One strategy in caries prevention is to inhibit the formation of cariogenic biofilms. Attempts are being made to develop oral hygiene products enriched with various antimicrobial agents. One of them is lactoperoxidase—an enzyme that can oxidise (pseudo)halide ions to reactive products with antimicrobial activity. Currently, commercially available products utilise thiocyanate as a substrate; however, several alternatives that are oxidised to products with greater antimicrobial potential have been found. In this study, toxicity against human gingival fibroblasts of the lactoperoxidase system was evaluated using four different (pseudo)halide substrate systems—thiocyanate, iodide, selenocyanate, and a mixture of thiocyanate and iodide. For this purpose, cells were treated with the systems and then apoptosis, cell cycle, intracellular glutathione concentration, and mitochondrial superoxide production were assessed. The results showed that each system, after generating 250 µM of the product, inhibited cell divisions, increased apoptosis, and increased the percentage of dead cells. It was concluded that the mechanism of the observed phenomena was not related to increased superoxide production or the depletion of glutathione concentration. These findings emphasised the need for the further in vitro and in vivo toxicity investigation of the modified lactoperoxidase system to assess its safety and the possibility of use in oral hygiene products.