Szczegóły publikacji
Opis bibliograficzny
Polyurethane/polylactide-blend films doped with zinc ions for the growth and expansion of human olfactory ensheathing cells (OECs) and adipose-derived mesenchymal stromal stem cells (ASCs) for regenerative medicine applications / Krzysztof Marycz, Monika Marędziak, Jakub Grzesiak, Dariusz Szarek, Anna LIS, Jadwiga LASKA // Polymers [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 2073-4360. — 2016 — vol. 8 iss. 5, s. 1–17. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 15–17, Abstr. — Publikacja dostępna online od: 2016-04-30
Autorzy (6)
- Marycz Krzysztof
- Marędziak Monika
- Grzesiak Jakub
- Szarek Dariusz
- AGHLis-Bartos Anna
- AGHLaska Jadwiga
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 98022 |
|---|---|
| Data dodania do BaDAP | 2016-06-09 |
| Tekst źródłowy | URL |
| DOI | 10.3390/polym8050175 |
| Rok publikacji | 2016 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Polymers (Basel) |
Abstract
Polymeric biomaterials based on polyurethane and polylactide blends are promising candidates for regenerative medicine applications as biocompatible, bioresorbable carriers. In current research we showed that 80/20 polyurethane/polylactide blends (PU/PLDL) with confirmed biological properties in vitro may be further improved by the addition of ZnO nanoparticles for the delivery of bioactive zinc oxide for cells. The PU/PLDL blends were doped with different concentrations of ZnO (0.001%, 0.01%, 0.05%) and undertaken for in vitro biological evaluation using human adipose stromal stem cells (ASCs) and olfactory ensheathing cells (OECs). The addition of 0.001% of ZnO to the biomaterials positively influenced the morphology, proliferation, and phenotype of cells cultured on the scaffolds. Moreover, the analysis of oxidative stress markers revealed that 0.001% of ZnO added to the material decreased the stress level in both cell lines. In addition, the levels of neural-specific genes were upregulated in OECs when cultured on sample 0.001 ZnO, while the apoptosis-related genes were downregulated in OECs and ASCs in the same group. Therefore, we showed that PU/PLDL blends doped with 0.001% of ZnO exert beneficial influence on ASCs and OECs in vitro and they may be considered for future applications in the field of regenerative medicine.
