Szczegóły publikacji
Opis bibliograficzny
Study of cellulolytic enzyme immobilization on copolymers of N-vinylformamide / Agnieszka TĄTA, Katarzyna Sokołowska, Joanna Świder, Anna Konieczna-Molenda, Edyta PRONIEWICZ, Ewa Witek // Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy ; ISSN 1386-1425. — 2015 — vol. 149, s. 494–504. — Bibliogr. s. 503–504, Abstr.
Autorzy (6)
- AGHTąta Agnieszka
- Sokołowska Katarzyna
- Świder J.
- Konieczna-Molenda Anna
- AGHProniewicz Edyta
- Witek E.
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 89350 |
|---|---|
| Data dodania do BaDAP | 2015-06-16 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.saa.2015.04.112 |
| Rok publikacji | 2015 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Spectrochimica Acta, Part A, Molecular and Biomolecular Spectroscopy |
Abstract
This study was focused on finding of effective carriers suitable for the immobilization of cellulase. Copolymers of N-vinylformamide (NW) and divinylbenzene (DVB) were synthesized by free radical crosslinking polymerization in inverse suspension. Methyl silicone oil was used as the continuous phase. Three polymeric carriers based on P(NVF-co-DVB) with varying degrees of crosslinking and spherical particles with different grain sizes were obtained. The formamide groups in these carriers were hydrolyzed to amino groups, yielding three P(VAm-co-DVB) polymers with vinylamine units. Enzyme, cellulase (Novozym (R) 476), was immobilized onto carriers with vinylamine (through glutaraldehyde) and vinylformamide groups (without glutaraldehyde). The efficiency of the enzyme immobilization was determined based on the enzymatic activity of the enzyme during the catalytic reaction relative to that of the native enzyme. All tested carriers were found to be effective carriers for the immobilization of cellulase. However, the catalytic activity of cellulase immobilized on the P(VAM-co-DVB0.27)/2000/350 carrier was higher than that for the native enzyme. In addition, two molecular spectroscopy methods, Fourier-transform absorption infrared spectroscopy (FT-IR) and Fourier-transform Raman spectroscopy (FT-Raman), were used to analyze the carriers. These studies provided complete information regarding the structure of the studied copolymers. (C) 2015 Elsevier B.V. All rights reserved.
