Szczegóły publikacji
Opis bibliograficzny
Micro-PIXE analyses of frozen-hydrated semi-thick biological sections / Y. D. Wang, J. Mesjasz-Przybyłowicz, G. Tylko, A. D. Barnabas, W. J. PRZYBYŁOWICZ // Nuclear Instruments & Methods in Physics Research. Section B, Beam interactions with materials and atoms ; ISSN 0168-583X. — 2013 — vol. 306, s. 134–139. — Bibliogr. s. 139, Abstr. — W. J. Przybyłowicz – dod. afiliacja: Materials Research Department, iThemba LABS. — 13th International Conference on Microscope Technology and Applications (ICNMTA 2012) : Lisbon, Portugal, 22–27 July, 2012
Autorzy (5)
- Wang Y. D.
- Mesjasz-Przybyłowicz Jolanta
- Tylko G.
- Barnabas Alban D.
- AGHPrzybyłowicz Wojciech Józef
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 78887 |
|---|---|
| Data dodania do BaDAP | 2014-01-16 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.nimb.2012.12.051 |
| Rok publikacji | 2013 |
| Typ publikacji | referat w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Nuclear Instruments & Methods in Physics Research, Section B, Beam Interactions with Materials and Atoms |
Abstract
Cryo-micro-PIXE system and methodology of microanalysis of frozen-hydrated semi-thick biological sections is described. A commercially available cryotransfer system used in electron microscopy has been adapted for this purpose. The analyzed material was frozen by metal-mirror method and sections of 20-50 micron thickness were prepared. Micro-PIXE and simultaneous proton backscattering was performed using 3 MeV proton beam. Monitoring of water vapour composition during the proton bombardment showed good stability of the analyzed material. The results of repetitive analyses of standards prepared from gelatin-glycerol solution with added known concentrations of K, Ni, Cu, Zn were in good agreement with expected, calculated values. Mass losses and changes of elemental composition were monitored. Elemental maps obtained for frozen-hydrated semi-thick section of Ni hyperaccumulator Senecio coronatus showed excellent preservation of leaf morphology and the distribution of elements. Quantitative elemental mapping of frozen-hydrated specimens compared with subsequent analysis of the same areas after freeze-drying revealed similar distribution pattern in both cases. It is clear, however, that freeze-drying induces some distortion of cell morphology and specimen shrinkage. (C) 2013 Elsevier B.V. All rights reserved.
