Szczegóły publikacji
Opis bibliograficzny
In-vacuum micro-PIXE analysis of biological specimens in frozen-hydrated state / G. Tylko, J. Mesjasz-Przybyłowicz, W. J. Przybyłowicz // Nuclear Instruments & Methods in Physics Research. Section B, Beam interactions with materials and atoms ; ISSN 0168-583X. — 2007 — vol. 260 iss. 1, s. 141–148. — Bibliogr. s. 147–148, Abstr. — Publikacja dostępna online od: 2017-02-14. — W. J. Przybyłowicz - pierwsza afiliacja: Materials Research Group, iThemba LABS, Somerset West, South Africa. — Nuclear Microprobe Technology and Applications (ICNMTA2006) and Proton Beam Writing (PBW II) ; 10th International Conference on Nuclear Microprobe Technology and Applications and 2nd International Workshop on Proton Beam Writing : Shangri La Rasa Resort, Singapore, 9–14 July 2006
Autorzy (3)
- Tylko Grzegorz
- Mesjasz-Przybyłowicz Jolanta
- AGHPrzybyłowicz Wojciech Józef
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 38645 |
|---|---|
| Data dodania do BaDAP | 2008-04-14 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.nimb.2007.02.017 |
| Rok publikacji | 2007 |
| Typ publikacji | referat w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Nuclear Instruments & Methods in Physics Research, Section B, Beam Interactions with Materials and Atoms |
Abstract
The first in-vacuum measurements of biological specimens in frozen-hydrated state using proton microbeam have been performed at Materials Research Group, iThemba LABS, South Africa. A commercially available cryotransfer system used in electron microscopy has been adapted for this purpose. The analyzed material was frozen in propane cooled by liquid nitrogen, fractured, carbon coated and transferred onto the cold stage (100 K) in the nuclear microprobe chamber. Micro-PIXE and simultaneous proton backscattering was performed using 3 MeV proton beam. Monitoring of water vapour composition during the proton bombardment showed good stability of the analyzed material. Quantitative results were obtained by standardless method, tested using 20% gelatine standards with added PbCl2. The differences between the concentrations obtained for frozen-hydrated and next freeze-dried specimens and the value calculated on the basis of weighted mass of PbCl2 added to gelatine were statistically insignificant. Leaf tissue of Senecio anomalochrous and larvae of Chysolina pardalina were used as examples of plant and animal tissue. Quantitative elemental mapping of frozen-hydrated specimens compared with subsequent analysis of the same areas after freeze-drying revealed the same distribution pattern in both cases.
