Szczegóły publikacji
Opis bibliograficzny
Bone marrow-derived macrophages loaded with boron carbide nanoparticles targeting the glioblastoma microenvironment for boron neutron capture therapy / Anna Rudawska, Agnieszka Szczygieł, Katarzyna Węgierek-Ciura, Jagoda Mierzejewska, Dawid KOZIEŃ, Paulina Żeliszewska, Monika Chaszczewska-Markowska, Piotr RUSINIAK, Katarzyna WĄTOR, Zbigniew PĘDZICH, Elżbieta Pajtasz-Piasecka, Bożena Szermer-Olearnik // International Journal of Nanomedicine [Dokument elektroniczny]. - Czasopismo elektroniczne ; ISSN 1178-2013 . — 2026 — vol. 21 art. no. 604151, s. 1–18. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 17–18, Abstr. — Publikacja dostępna online od: 2026-06-03
Autorzy (12)
- Rudawska Anna
- Szczygieł Agnieszka
- Węgierek-Ciura Katarzyna
- Mierzejewska Jagoda
- AGHKozień Dawid
- Żeliszewska Paulina
- Chaszczewska-Markowska Monika
- AGHRusiniak Piotr
- AGHWątor Katarzyna
- AGHPędzich Zbigniew
- Pajtasz-Piasecka Elżbieta
- Szermer-Olearnik Bożena
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 168319 |
|---|---|
| Data dodania do BaDAP | 2026-07-07 |
| Tekst źródłowy | URL |
| DOI | 10.2147/IJN.S604151 |
| Rok publikacji | 2026 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | International Journal of Nanomedicine |
Abstract
Introduction: Boron neutron capture therapy (BNCT) is a targeted radiotherapy that represents a promising treatment for glioblastoma multiforme. BNCT efficacy depends on selective boron carriers capable of crossing the blood–brain barrier (BBB). Therefore, we propose an original strategy for boron delivery in BNCT, based on the cellular carriers. This study assessed the ability of bone marrow-derived macrophages (BMDMs) to accumulate boron carbide (B4C) nanoparticles, transport them across an in vitro BBB model toward glioblastoma cell-conditioned medium, and interact with tumor cells. Methods: The uptake and accumulation of B4C nanoparticles by BMDMs in different polarization states (M0, M1, M2) were confirmed by holotomography and ICP-MS. The scratch assay assessed spontaneous migration of BMDMs loaded with B4C nanoparticles. The Transwell system was used to determine the ability of BMDMs with B4C to cross bEnd.3 brain endothelial cell monolayer, mimicking the BBB, toward GL-261 glioblastoma cell-conditioned medium rich in CCL2. Macrophage interactions with glioblastoma spheroids were investigated using the CellTiter-Glo 3D Cell Viability Assay and flow cytometry. Results: All BMDM populations demonstrated the ability to accumulate B4C nanoparticles, with the significantly highest boron concentration detected in M1 macrophages (21.53 ± 1.64 mg/L per 106 cells). Moreover, M0, M1, and M2 macrophages loaded with B4C nanoparticles were capable of migrating through the brain endothelial cell monolayer, toward the glioblastoma cell-conditioned medium. BMDMs with nanoparticles did not affect the viability of glioblastoma spheroids after 6 days of co-culture, in contrast to macrophages without nanoparticles, which increased the survival of tumor cells. Importantly, CD206 expression was not increased in M1 macrophages after contact with spheroids, and was lower in M2 macrophages loaded with B4C nanoparticles compared to control M2 macrophages. Conclusion: BMDMs are promising carriers of B4C nanoparticles for BNCT due to their ability to cross the in vitro BBB model toward the glioblastoma microenvironment.
