Szczegóły publikacji
Opis bibliograficzny
Keratin-TMAO dressing accelerates full-thickness skin wound healing in diabetic rats via M2-macrophage polarization and the activation of PI3K/AKT/mTOR signaling pathway / Mateusz Rybka, Łukasz Mazurek, Jan Jurak, Anna Laskowska, Mikołaj Zajdel, Joanna Czuwara, Dorota Sulejczak, Mateusz Szudzik, Emilia Samborowska, Robert A. Schwartz, Michał DZIADEK, Szymon SALAGIERSKI, Adrian Drapała, Marcin Ufnal, Marek Konop // International Journal of Biological Macromolecules ; ISSN 0141-8130. — 2025 — vol. 310 pt. 3 art. no. 143313, s. 1–13. — Bibliogr. s. 11–13, Abstr. — Publikacja dostępna online od: 2025-04-22. — M. Dziadek - dod. afiliacja: University of British Columbia, Vancouver, British Columbia, Canada
Autorzy (15)
- Rybka Mateusz
- Mazurek Łukasz
- Jurak Jan
- Laskowska Anna K.
- Zajdel Mikołaj
- Czuwara Joanna
- Sulejczak Dorota
- Szudzik Mateusz
- Samborowska Emilia
- Schwartz Robert A.
- AGHDziadek Michał
- AGHSalagierski Szymon
- Drapała Adrian
- Ufnal Marcin
- Konop Marek
Dane bibliometryczne
| ID BaDAP | 159780 |
|---|---|
| Data dodania do BaDAP | 2025-06-04 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.ijbiomac.2025.143313 |
| Rok publikacji | 2025 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | International Journal of Biological Macromolecules |
Abstract
Impaired wound healing reduces quality of life, increases the risk of infection and often shortens lifespan. We developed a novel wound dressing based on fur keratin derived powder (FKDP) enriched with trimethylamine N-oxide (TMAO) and evaluated its efficacy in vitro and in vivo. In vitro, a 0.1 % (m/v) FKDP+TMAO suspension increased keratinocyte viability by up to 44.9 % ± 8.6 % (p < 0.05) and enhanced PI3K/AKT/mTOR pathway activation as indicated by a 2.6-fold increase in p-RPS6 expression (p < 0.05), which is a surrogate marker of PI3K/AKT/mTOR activation. Real-time PCR studies showed up-regulation of KRT17 in cells treated with FKDP+TMAO suspension (p < 0.05). In vivo, full-thickness wounds in diabetic rats (n = 30) treated with FKDP+TMAO showed significantly faster healing rate on days 4 (40.1 % ± 14.4 % vs. 29.1 % ± 16.9 %, p < 0.01), 7 (64.5 % ± 17.2 % vs. 56.9 % ± 21.2 %, p < 0.01) and 14 (95.9 % ± 5.0 % vs. 90.6 % ± 11.0 %, p < 0.05). All treated wounds healed by day 21, while untreated wounds still remained partially unhealed (98.7 % ± 1.9 %, p < 0.05). Immunohistochemical analysis revealed that treated wounds were characterized by an earlier infiltration of pro-healing M2 macrophages, whereas at the same time point in control wounds pro-inflammatory M1 macrophages predominated (p < 0.05). These findings suggest that FKDP+TMAO is a safe and effective wound dressing that accelerates healing through immunoregulation and activation of the PI3K/AKT/mTOR pathway.
