Szczegóły publikacji
Opis bibliograficzny
Evaluation of changes in tissue material during fixation and formation processes using total Reflection X-ray Fluorescence Spectrometry / Paula KASPRZYK, Paweł M. WRÓBEL, Łukasz Chmura, Konrad Kozłowski, Katarzyna WĄTOR, Magdalena SZCZERBOWSKA-BORUCHOWSKA // Microchemical Journal ; ISSN 0026-265X. — 2025 — vol. 212 art. no. 113348, s. 1–7. — Bibliogr. s. 7, Abstr. — Publikacja dostępna online od: 2025-03-20
Autorzy (6)
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 159314 |
|---|---|
| Data dodania do BaDAP | 2025-05-20 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.microc.2025.113348 |
| Rok publikacji | 2025 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Microchemical Journal |
Abstract
The balance of macro and trace elements is vital to the functionality of biological systems, with significant deviations affecting cellular processes. Elemental analysis in tissues can offer insights into pathological mechanisms and identify diagnostic markers. However, while frozen samples are traditionally preferred for such studies, formalin-fixed and paraffin-embedded (FFPE) tissues are often used due to ease of storage and access, although the effects of fixation on elemental composition are not well understood. This study investigates the impact of FFPE processing on the elemental composition of swine liver, spleen, and muscle tissues, using freeze-dried samples as a control for comparative analysis. Total Reflection X-Ray Fluorescence (TXRF) spectroscopy revealed substantial changes in elemental concentrations post-processing. Alkali metals, particularly potassium and rubidium, showed significant reductions, with pronounced leaching occurring within the first six hours of fixation. Conversely, alkaline earth metals like calcium exhibited increased concentrations. Notable reproducibility challenges were encountered, particularly for liver tissues, suggesting that FFPE processing introduces variability. Despite these limitations, the study demonstrates that large-scale elemental analyses of FFPE tissues can be feasible with advanced analytical techniques. The findings underscore the novel observation of time-dependent elemental shifts during formalin fixation, emphasizing the importance of optimized sample preparation in such studies. These insights contribute to a more reliable basis for large-scale research using archived tissue samples and may enhance diagnostic and pathological investigations.
