Szczegóły publikacji

Opis bibliograficzny

Preparation of tissue samples for X-ray fluorescence microscopy / Joanna CHWIEJ, Magdalena SZCZERBOWSKA-BORUCHOWSKA, Marek LANKOSZ, Sławomir WÓJCIK, Gerald Falkenberg, Zdzisław STĘGOWSKI, Zuzanna Setkowicz // Spectrochimica Acta. Part B, Atomic spectroscopy ; ISSN 0584-8547. — 2005 — vol. 60 iss. 12, s. 1531–1537. — Bibliogr. s. 1536–1537, Abstr.


Autorzy (7)


Słowa kluczowe

paraffintissue sample preparationparaformaldehydeformalinsynchrotron microbeam X-ray fluorescenceX-ray fluorescence microscopy

Dane bibliometryczne

ID BaDAP25291
Data dodania do BaDAP2006-01-02
Tekst źródłowyURL
DOI10.1016/j.sab.2005.10.002
Rok publikacji2005
Typ publikacjiartykuł w czasopiśmie
Otwarty dostęptak
Czasopismo/seriaSpectrochimica Acta, Part B, Atomic Spectroscopy

Abstract

As is well-known, trace elements, especially metals, play an important role in the pathogenesis of many disorders. The topographic and quantitative elemental analysis of pathologically changed tissues may shed some new light on processes leading to the degeneration of cells in the case of selected diseases. An ideal and powerful tool for such purpose is the Synchrotron Microbeam X-ray Fluorescence technique. It enables the carrying out of investigations of the elemental composition of tissues even at the single cell level. The tissue samples for histopathological investigations are routinely fixed and embedded in paraffin. The authors try to verify the usefulness of such prepared tissue sections for elemental analysis with the use of X-ray fluorescence microscopy. Studies were performed on rat brain samples. Changes in elemental composition caused by fixation in formalin or paraformaldehyde and embedding in paraffin were examined. Measurements were carried out at the bending magnet beamline L of the Hamburger Synchrotronstrahlungslabor HASYLAB in Hamburg. The decrease in mass per unit area of K, Br and the increase in P, S, Fe, Cu and Zn in the tissue were observed as a result of the fixation. For the samples embedded in paraffin, a lower level of most elements was observed. Additionally, for these samples, changes in the composition of some elements were not uniform for different analyzed areas of rat brain.

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