Szczegóły publikacji
Opis bibliograficzny
Cellular response to bone morphogenetic proteins-2 and -7 covalently bound to photocrosslinked heparin–diazoresin multilayer / Magdalena WYTRWAŁ, Małgorzata Sekuła-Stryjewska, Agata Pomorska, Ewa Oclon, Ewa Zuba-Surma, Szczepan ZAPOTOCZNY, Krzysztof Szczubiałka // Biomolecules [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 2218-273X. — 2023 — vol. 13 iss. 5 art. no. 842, s. 1–17. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 15–17, Abstr. — Publikacja dostępna online od: 2023-05-15. — Sz. Zapotoczny - dod. afiliacja: Faculty of Chemistry, Jagiellonian University, Krakow
Autorzy (7)
- AGHWytrwał Magdalena
- Sekuła-Stryjewska Małgorzata
- Pomorska Agata
- Oclon Ewa
- Zuba-Surma Ewa
- AGHZapotoczny Szczepan
- Szczubiałka Krzysztof
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 146820 |
|---|---|
| Data dodania do BaDAP | 2023-05-29 |
| Tekst źródłowy | URL |
| DOI | 10.3390/biom13050842 |
| Rok publikacji | 2023 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Biomolecules |
Abstract
Despite the plethora of research that exists on recombinant human bone morphogenetic protein-2 and -7 (rhBMP-2 and rhBMP-7) and has been clinically approved, there is still a need to gain information that would allow for their more rational use in bone implantology. The clinical application of supra-physiological dosages of these superactive molecules causes many serious adverse effects. At the cellular level, they play a role in osteogenesis and cellular adhesion, migration, and proliferation around the implant. Therefore, in this work, we investigated the role of the covalent binding of rhBMP-2 and rhBMP-7 separately and in combination with ultrathin multilayers composed of heparin and diazoresin in stem cells. In the first step, we optimized the protein deposition conditions via quartz crystal microbalance (QCM). Then, atomic force microscopy (AFM) and enzyme-linked immunosorbent assay (ELISA) were used to analyze protein–substrate interactions. The effect of the protein binding on the initial cell adhesion, migration, and short-term expression of osteogenesis markers was tested. In the presence of both proteins, cell flattening and adhesion became more prominent, resulting in limited motility. However, the early osteogenic marker expression significantly increased compared to the single protein systems. The presence of single proteins resulted in the elongation of cells, which promoted their migration activity.