Szczegóły publikacji
Opis bibliograficzny
The effect of the topmost layer and the type of bone morphogenetic protein-2 immobilization on the mesenchymal stem cell response / Magdalena WYTRWAŁ-SARNA, Małgorzata Sekuła-Stryjewska, Agata Pomorska, Ewa Ocłoń, Katarzyna Gajos, Michał Sarna, Ewa Zuba-Surma, Andrzej BERNASIK, Krzysztof Szczubiałka // International Journal of Molecular Sciences [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 1422-0067. — 2022 — vol. 23 iss. 16 art. no. 9287, s. 1–19. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 17–19, Abstr. — Publikacja dostępna online od: 2022-08-18
Autorzy (9)
- AGHWytrwał Magdalena
- Sekuła-Stryjewska Małgorzata
- Pomorska Agata
- Ocłoń Ewa
- Gajos Katarzyna
- Sarna Michał
- Zuba-Surma Ewa
- AGHBernasik Andrzej
- Szczubiałka Krzysztof
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 141676 |
|---|---|
| Data dodania do BaDAP | 2022-09-05 |
| Tekst źródłowy | URL |
| DOI | 10.3390/ijms23169287 |
| Rok publikacji | 2022 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | International Journal of Molecular Sciences |
Abstract
Recombinant human bone morphogenetic protein-2 (rhBMP-2) plays a key role in the stem cell response, not only via its influence on osteogenesis, but also on cellular adhesion, migration, and proliferation. However, when applied clinically, its supra-physiological levels cause many adverse effects. Therefore, there is a need to concomitantly retain the biological activity of BMP-2 and reduce its doses. Currently, the most promising strategies involve site-specific and site-directed immobilization of rhBMP-2. This work investigated the covalent and electrostatic binding of rhBMP-2 to ultrathin-multilayers with chondroitin sulfate (CS) or diazoresin (DR) as the topmost layer. Angle-resolved X-ray photoelectron spectroscopy was used to study the exposed chemical groups. The rhBMP-2 binding efficiency and protein state were studied with time-of-flight secondary ion mass spectrometry. Quartz crystal microbalance, atomic force microscopy, and enzyme-linked immunosorbent assay were used to analyze protein–substrate interactions. The effect of the topmost layer was tested on initial cell adhesion and short-term osteogenesis marker expression. The results show the highest expression of selected osteomarkers in cells cultured on the DR-ended layer, while the cellular flattening was rather poor compared to the CS-ended system. rhBMP-2 adhesion was observed only on negatively charged layers. Cell flattening became more prominent in the presence of the protein, even though the osteogenic gene expression decreased.