Szczegóły publikacji
Opis bibliograficzny
In vitro cytocompatibility and antibacterial studies on biodegradable Zn alloys supplemented by a critical assessment of direct contact cytotoxicity assay / Maria WĄTROBA, Wiktor Bednarczyk, Piotr K. SZEWCZYK, Jakub KAWAŁKO, Krzysztof MECH, Alina Grünewald, Irem Unalan, Nicola Taccardi, Gabriela Boelter, Manuel Banzhaf, Caroline Hain, Piotr BAŁA, Aldo R. Boccaccini // Journal of Biomedical Materials Research. Part B, Applied Biomaterials ; ISSN 1552-4973. — 2023 — vol. 111 iss. 2, s. 241–260. — Bibliogr. s. 258–260, Abstr. — Publikacja dostępna online od: 2022-08-26. — M. Wątroba - dod. afiliacja: Empa, Swiss Federal Laboratories for Materials Science and Technology, Thun, Switzerland; P. Bała - dod. afiliacja: ACMiN AGH; P. Szewczyk - w publikacji podano błędny nr ORCID
Autorzy (13)
- AGHWątroba Maria
- Bednarczyk Wiktor
- AGHSzewczyk Piotr K.
- AGHKawałko Jakub
- AGHMech Krzysztof
- Grünewald Alina
- Unalan Irem
- Taccardi Nicola
- Boelter Gabriela
- Banzhaf Manuel
- Hain Caroline
- AGHBała Piotr
- Boccaccini Aldo Roberto
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 144183 |
|---|---|
| Data dodania do BaDAP | 2022-12-22 |
| Tekst źródłowy | URL |
| DOI | 10.1002/jbm.b.35147 |
| Rok publikacji | 2023 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Journal of Biomedical Materials Research, Part B, Applied Biomaterials |
Abstract
In vitro cytotoxicity assessment is indispensable in developing new biodegradable implant materials. Zn, which demonstrates an ideal corrosion rate between Mg- and Fe-based alloys, has been reported to have excellent in vivo biocompatibility. Therefore, modifications aimed at improving Zn's mechanical properties should not degrade its biological response. As sufficient strength, ductility and corrosion behavior required of load-bearing implants has been obtained in plastically deformed Zn-3Ag-0.5Mg, the effect of simultaneous Ag and Mg additions on in vitro cytocompatibility and antibacterial properties was studied, in relation to Zn and Zn-3Ag. Direct cell culture on samples and indirect extract-based tests showed almost no significant differences between the tested Zn-based materials. The diluted extracts of Zn, Zn-3Ag, and Zn-3Ag-0.5Mg showed no cytotoxicity toward MG-63 cells at a concentration of <= 12.5%. The cytotoxic effect was observed only at high Zn2+ ion concentrations and when in direct contact with metallic samples. The highest LD50 (lethal dose killing 50% of cells) of 13.4 mg/L of Zn2+ ions were determined for the Zn-3Ag-0.5Mg. Similar antibacterial activity against Escherichia coli and Staphylococcus aureus was observed for Zn and Zn alloys, so the effect is attributed mainly to the released Zn2+ ions exhibiting bactericidal properties. Most importantly, our experiments indicated the limitations of water-soluble tetrazolium salt-based cytotoxicity assays for direct tests on Zn-based materials. The discrepancies between the WST-8 assay and SEM observations are attributed to the interference of Zn2+ ions with tetrazolium salt, therefore favoring its transformation into formazan, giving false cell viability quantitative results.
