Szczegóły publikacji
Opis bibliograficzny
A study of the interactions between human osteoblast-like cells and polymer composites with functionalized graphene derivatives using 2D correlation spectroscopy (2D-COS) / Anna Kołodziej, Aleksandra Wesełucha-Birczyńska, Elżbieta DŁUGOŃ, Małgorzata Świętek, Maciej GUBERNAT, Łukasz Skalniak, Marta BŁAŻEWICZ // Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy ; ISSN 1386-1425. — 2023 — vol. 285 art. no. 121862, s. 1–8. — Bibliogr. s. 7–8, Abstr. — Publikacja dostępna online od: 2022-09-10
Autorzy (7)
- Kołodziej Anna
- Wesełucha-Birczyńska Aleksandra
- AGHDługoń Elżbieta
- Świętek Małgorzata
- AGHGubernat Maciej
- Skalniak Łukasz
- AGHBłażewicz Marta
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 143183 |
|---|---|
| Data dodania do BaDAP | 2022-10-21 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.saa.2022.121862 |
| Rok publikacji | 2023 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Spectrochimica Acta, Part A, Molecular and Biomolecular Spectroscopy |
Abstract
In response to the growing need for development of modern biomaterials for applications in regenerative medicine strategies, the research presented here investigated the biological potential of two types of polymer nanocomposites. Graphene oxide (GO) and partially reduced graphene oxide (rGO) were incorporated into a poly(ε-caprolactone) (PCL) matrix, creating PCL/GO and PCL/rGO nanocomposites in the form of membranes. Proliferation of osteoblast-like cells (human U-2 OS cell line) on the surface of the studied materials confirmed their biological activity. Fluorescence microscopy was able to distinguish the different patterns of interaction between cells (depending on the type of material) after 15 days of the test run. Raman micro-spectroscopy and two-dimensional correlation spectroscopy (2D-COS) applied to Raman spectra distinguished the nature of cell-material interactions after only 8 days. Combination of these two techniques (Raman micro-spectroscopy and 2D-COS analysis) facilitated identification of a much more complex cellular response (especially from proteins) on the surface of PCL/GO. The presented approach can be regarded as a method for early study of the bioactivity of membrane materials.
