Szczegóły publikacji
Opis bibliograficzny
Promotion through external magnetic field of osteogenic differentiation potential in adipose-derived mesenchymal stem cells: design of polyurethane/poly(lactic) acid sponges doped with iron oxide nanoparticles / Krzysztof Marycz, Michalina Alicka, Katarzyna Kornicka-Garbowska, Joanna POLNAR, Anna LIS-BARTOS, Rafał J. Wiglusz, Michael Roecken, Jean-Marie Nedelec // Journal of Biomedical Materials Research. Part B, Applied Biomaterials ; ISSN 1552-4973. — 2020 — vol. 108 iss. 4, s. 1398–1411. — Bibliogr. s. 1410–1411, Abstr. — Publikacja dostępna online od: 2019-09-12
Autorzy (8)
- Marycz Krzysztof
- Alicka Michalina
- Kornicka-Grabowska Katarzyna
- AGHPolnar Joanna
- AGHLis-Bartos Anna
- Wiglusz Rafał J.
- Roecken Michael
- Nedelec Jean-Marie
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 128413 |
|---|---|
| Data dodania do BaDAP | 2020-04-22 |
| Tekst źródłowy | URL |
| DOI | 10.1002/jbm.b.34488 |
| Rok publikacji | 2020 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Journal of Biomedical Materials Research, Part B, Applied Biomaterials |
Abstract
Recently, iron oxide nanoparticles (IONPs) have gathered special attention in regenerative medicine. Owing to their magnetic and bioactive properties, IONPs are utilized in the fabrication of novel biomaterials. Yet, there was no report regarding thermoplastic polyurethane (TPU) and poly(lactic acid) (PLA) polymer doped with IONPs on osteogenic differentiation of mesenchymal stem cells. Thus the objectives of presented study was to: (a) fabricate magnetic TPU + PLA sponges doped with iron (III) oxide Fe2O3 nanoparticles; (b) investigate the effects of biomaterial and its exposition to static magnetic field (MF) on osteogenic differentiation, proliferation, and apoptosis in adipose‐derived mesenchymal stem cells (ASCs). TPU + PLA sponges were prepared using solvent casting technique while incorporation of the Fe2O3 nanoparticles was performed with solution cast method. RT‐PCR was applied to evaluate expression of osteogenic‐related genes and integrin's in cells cultured on fabricated materials with or without the stimulation of static MF. MF stimulation enhanced the expression of osteopontin and collagen type I while decreased expression of bone morphogenetic protein 2 in tested magnetic materials—TPU + PLA/1% Fe2O3 and TPU + PLA/5% Fe2O3. Therefore, TPU + PLA sponges doped with IONPs and exposure to MF resulted in improved osteogenic differentiation of ASC.
