Szczegóły publikacji

Opis bibliograficzny

Autorzy (8)

• Marycz Krzysztof
• Alicka Michalina
• Kornicka-Grabowska Katarzyna
• AGHPolnar Joanna
• AGHLis-Bartos Anna
• Wiglusz Rafał J.
• Roecken Michael
• Nedelec Jean-Marie

Słowa kluczowe

Dane bibliometryczne

ID BaDAP	128413
Data dodania do BaDAP	2020-04-22
Tekst źródłowy	URL
DOI	10.1002/jbm.b.34488
Rok publikacji	2020
Typ publikacji	artykuł w czasopiśmie
Otwarty dostęp
Czasopismo/seria	Journal of Biomedical Materials Research, Part B, Applied Biomaterials

Abstract

Recently, iron oxide nanoparticles (IONPs) have gathered special attention in regenerative medicine. Owing to their magnetic and bioactive properties, IONPs are utilized in the fabrication of novel biomaterials. Yet, there was no report regarding thermoplastic polyurethane (TPU) and poly(lactic acid) (PLA) polymer doped with IONPs on osteogenic differentiation of mesenchymal stem cells. Thus the objectives of presented study was to: (a) fabricate magnetic TPU + PLA sponges doped with iron (III) oxide Fe2O3 nanoparticles; (b) investigate the effects of biomaterial and its exposition to static magnetic field (MF) on osteogenic differentiation, proliferation, and apoptosis in adipose‐derived mesenchymal stem cells (ASCs). TPU + PLA sponges were prepared using solvent casting technique while incorporation of the Fe2O3 nanoparticles was performed with solution cast method. RT‐PCR was applied to evaluate expression of osteogenic‐related genes and integrin's in cells cultured on fabricated materials with or without the stimulation of static MF. MF stimulation enhanced the expression of osteopontin and collagen type I while decreased expression of bone morphogenetic protein 2 in tested magnetic materials—TPU + PLA/1% Fe2O3 and TPU + PLA/5% Fe2O3. Therefore, TPU + PLA sponges doped with IONPs and exposure to MF resulted in improved osteogenic differentiation of ASC.

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