Szczegóły publikacji
Opis bibliograficzny
Measurement of multi ion transport through human bronchial epithelial cell line provides an insight into the mechanism of defective water transport in cystic fibrosis / Mirosław Zając, Andrzej LEWENSTAM, Piotr Bednarczyk, Krzysztof Dołowy // Membranes [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 2077-0375. — 2020 — vol. 10 iss. 3 art. no. 43, s. 1–13. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 11–13, Abstr. — Publikacja dostępna online od: 2020-03-12. — A. Lewenstam - dod. afiliacja: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw
Autorzy (4)
- Zając Mirosław
- AGHLewenstam Andrzej
- Bednarczyk Piotr
- Dołowy Krzysztof
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 128207 |
|---|---|
| Data dodania do BaDAP | 2020-04-06 |
| Tekst źródłowy | URL |
| DOI | 10.3390/membranes10030043 |
| Rok publikacji | 2020 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Membranes |
Abstract
We measured concentration changes of sodium, potassium, chloride ions, pH and the transepithelial potential difference by means of ion-selective electrodes, which were placed on both sides of a human bronchial epithelial 16HBE14σ cell line grown on a porous support in the presence of ion channel blockers. We found that, in the isosmotic transepithelial concentration gradient of either sodium or chloride ions, there is an electroneutral transport of the isosmotic solution of sodium chloride in both directions across the cell monolayer. The transepithelial potential difference is below 3 mV. Potassium and pH change plays a minor role in ion transport. Based on our measurements, we hypothesize that in a healthy bronchial epithelium, there is a dynamic balance between water absorption and secretion. Water absorption is caused by the action of two exchangers, Na/H and Cl/HCO3, secreting weakly dissociated carbonic acid in exchange for well dissociated NaCl and water. The water secretion phase is triggered by an apical low volume-dependent factor opening the Cystic Fibrosis Transmembrane Regulator CFTR channel and secreting anions that are accompanied by paracellular sodium and water transport. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.