Szczegóły publikacji
Opis bibliograficzny
Multi-electrode system for measurement of transmembrane ion-fluxes through living epithelial cells / Mirosław Zając, Andrzej LEWENSTAM, Krzysztof Dołowy // Bioelectrochemistry / Bioelectrochemical Society ; ISSN 1567-5394. — Tytuł poprz.: Bioelectrochemistry and Bioenergetics ; ISSN: 0302-4598. — 2017 — vol. 117, s. 65–73. — Bibliogr. s. 72–73, Abstr. — Publikacja dostępna online od:2017-06-13. — A. Lewenstam - pierwsza afiliacja: Åbo Akademi University, Finland
Autorzy (3)
- Zając Mirosław
- AGHLewenstam Andrzej
- Dołowy Krzysztof
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 107041 |
|---|---|
| Data dodania do BaDAP | 2017-09-01 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.bioelechem.2017.06.007 |
| Rok publikacji | 2017 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Bioelectrochemistry |
Abstract
Cystic Fibrosis (CF) is the most common fatal human genetic disease. It is caused by the defect in a single anion channel protein which affects ion and water transport across the epithelial tissue. A flat multi-electrode platform of diameter 12 mm, allowing for measurement of four ions: sodium, potassium, hydrogen and chloride by exchangeable/replaceable ion-selective electrodes is described. The measurement is possible owing to the architecture of the platform which accommodates all the electrodes and inlets/outlets. The platform fits to the cup and operates in a small volume of the solution bathing the living epithelial cell layer (membrane) deposited on a porous support of the cup, which allows for effective monitoring of ion concentration changes. By applying two multi-electrode platforms, it is possible to measure the ion transmembrane fluxes. The inlet and outlet tubes in the platforms allow for on-fly change of the calibrants, ion-concentration changes and ion channel blockers. Using different ion-concentration gradients and blockers of ion-transporting molecules we show for the first time that sodium ions flow from the basolateral to apical face of the cell monolayer via a paracellular route and return also via a transcellular one, while chloride anions are transported back and forth exclusively via a transcellular route.