Szczegóły publikacji
Opis bibliograficzny
Fabrication, characterization, and cytotoxicity of thermoplastic polyurethane/poly(lactic acid) material using human adipose derived mesenchymal stromal stem cells (hASCs) / Anna LIS-BARTOS, Agnieszka Smieszek, Kinga Frańczyk, Krzysztof Marycz // Polymers [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 2073-4360. — 2018 — vol. 10 iss. 10 art. no. 1073, s. 1–14. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 12–14, Abstr. — Publikacja dostępna online od: 2018-09-28. — A. Lis-Bartos - dod. afiliacja: Wrocław University of Environmental and Life Sciences
Autorzy (4)
- AGHLis-Bartos Anna
- Śmieszek Agnieszka
- AGHFrańczyk Kinga
- Marycz Krzysztof
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 118468 |
|---|---|
| Data dodania do BaDAP | 2018-12-11 |
| Tekst źródłowy | URL |
| DOI | 10.3390/polym10101073 |
| Rok publikacji | 2018 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Polymers (Basel) |
Abstract
Thermoplastic polyurethane (TPU) and poly(lactic acid) are types of biocompatible and degradable synthetic polymers required for biomedical applications. Physically blended (TPU+PLA) tissue engineering matrices were produced via solvent casting technique. The following types of polymer blend were prepared: (TPU+PLA) 7:3, (TPU+PLA) 6:4, (TPU+PLA) 4:6, and (TPU+PLA) 3:7. Various methods were employed to characterize the properties of these polymers: surface properties such as morphology (scanning electron microscopy), wettability (goniometry), and roughness (profilometric analysis). Analyses of hydrophilic and hydrophobic properties, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) of the obtained polymer blends were conducted. Tensile tests demonstrated that the blends exhibited a wide range of mechanical properties. Cytotoxicity of polymers was tested using human multipotent stromal cells derived from adipose tissue (hASC). In vitro assays revealed that (TPU+PLA) 3:7 matrices were the most cytocompatible biomaterials. Cells cultured on (TPU+PLA) 3:7 had proper morphology, growth pattern, and were distinguished by increased proliferative and metabolic activity. Additionally, it appeared that (TPU+PLA) 3:7 biomaterials showed antiapoptotic properties. hASC cultured on these matrices had reduced expression of Bax-alpha and increased expression of Bcl-2. This study demonstrated the feasibility of producing a biocompatible scaffold form based on (TPU+PLA) blends that have potential to be applied in tissue engineering.
