Szczegóły publikacji

Opis bibliograficzny

Autorzy (6)

• Kolasińska-Sojka Marta
• Włodek Magdalena
• AGHSzuwarzyński Michał
• Kereiche Sami
• Kovacik Lubomir
• Warszyński Piotr

Słowa kluczowe

Dane bibliometryczne

ID BaDAP	111212
Data dodania do BaDAP	2018-01-11
Tekst źródłowy	URL
DOI	10.1016/j.colsurfb.2017.07.046
Rok publikacji	2017
Typ publikacji	artykuł w czasopiśmie
Otwarty dostęp
Czasopismo/seria	Colloids and Surfaces, B, Biointerfaces

Abstract

The formation and properties of supported lipid bilayers (SLB) containing hydrophobic nanoparticles (NP) was studied in relation to underlying cushion obtained from selected polyelectrolyte multilayers. Lipid vesicles were formed from zwitterionic 1-palmitoy1-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and negatively charged 1-palmitoy1-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) in phosphate buffer (PBS). As hydrophobic nanoparticles quantum dots (QD) with size of 3.8 nm (emission wavelength of 420 nm) were used. Polyelectrolyte multilayers (PEM) were constructed by the sequential, i.e., layer-by-layer (LbL) adsorption of alternately charged polyelectrolytes from their solutions. Liposomes and Liposome-QDs complexes were studied with Transmission Cryo-Electron Microscopy (Cryo-TEM) to verify the quality of vesicles and the position of QD within lipid bilayer. Deposition of liposomes and liposomes with quantum dots on polyelectrolyte films was studied in situ using quartz crystal microbalance with dissipation (QCM-D) technique. The fluorescence emission spectra were analyzed for both: suspension of liposomes with nanoparticles and for supported lipid bilayers containing QD on PEM. It was demonstrated that quantum dots are located in the hydrophobic part of lipid bilayer. Moreover, we proved that such QD-modified liposomes formed supported lipid bilayers and their final structure depended on the type of underlying cushion.

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