Szczegóły publikacji
Opis bibliograficzny
Surface-enhanced Raman studies of bradykinin using colloidal gold / Dominika ŚWIĘCH, Younkyoo Kim, Edyta PRONIEWICZ // Vibrational Spectroscopy ; ISSN 0924-2031. — 2016 — vol. 83, s. 101–107. — Bibliogr. s. 106–107, Abstr. — Publikacja dostępna online od: 2016-02-02
Autorzy (3)
- AGHŚwięch Dominika
- Kim Younkyoo
- AGHProniewicz Edyta
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 96209 |
|---|---|
| Data dodania do BaDAP | 2016-02-16 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.vibspec.2016.01.014 |
| Rok publikacji | 2016 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Vibrational Spectroscopy |
Abstract
In this paper, bradykinin (BK), an endogenous peptide hormone, which is involved in a number of physiological and pathophysiological processes was deposited onto the colloidal Au nanoparticles. The surface-enhanced Raman spectroscopy (SERS) was used to determine the adsorption mode of BK under different environmental conditions, including: excitation wavelengths (514.5 nm and 785.0 nm), pH of aqueous sol solutions (from pH = 3 to pH = 11), and size of the colloidal nanoparticles (10, 20, and 50 nm). The metal surface plasmon of the colloidal suspended Au nanoparticles was examined by ultraviolet-visible (UV–vis) spectroscopy. The results showed that the C-terminal part of BK plays a crucial role in the adsorption process onto the colloidal suspended Au particles. The Phe5/8 and Arg9 residues of BK mainly participate in the interactions with the colloidal Au nanoparticles. At acidic pH of the solution (pH = 3), the BK single bondCOO− terminal group through the both oxygen atoms strongly binds to the Au nanoparticles. The Phe5/Phe8 rings adopt tilted orientation with respect to the colloidal Au nanoparticles with diameters of 10 and 20 nm. As the particle size increases to 50 nm, the flat orientation of the Phe ring(s) with respect to the Au nanoparticles is observed.
