Szczegóły publikacji
Opis bibliograficzny
Flowing atmospheric pressure afterglow combined with laser ablation for direct analysis of compounds separated by thin-layer chromatography / Michał Cegłowski, Marek SMOLUCH, Edward Reszke, Jerzy SILBERRING, Grzegorz Schroeder // Analytical and Bioanalytical Chemistry ; ISSN 1618-2642. — 2016 — vol. 408 iss. 3, s. 815–823. — Bibliogr. s. 822–823, Abstr. — Publikacja dostępna online od: 2015-11-12. — J. Silberring – dod. afiliacja: Polish Academy of Sciences
Autorzy (5)
- Cegłowski Michał
- AGHSmoluch Marek
- Reszke Edward
- AGHSilberring Jerzy
- Schroeder Grzegorz
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 95623 |
|---|---|
| Data dodania do BaDAP | 2016-02-03 |
| Tekst źródłowy | URL |
| DOI | 10.1007/s00216-015-9165-5 |
| Rok publikacji | 2016 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Analytical and Bioanalytical Chemistry |
Abstract
A thin-layer chromatography-mass spectrometry (TLC-MS) setup for characterization of low molecular weight compounds separated on standard TLC plates has been constructed. This new approach successfully combines TLC separation, laser ablation, and ionization using flowing atmospheric pressure afterglow (FAPA) source. For the laser ablation, a low-priced 445-nm continuous-wave diode laser pointer, with a power of 1 W, was used. The combination of the simple, low-budget laser pointer and the FAPA ion source has made this experimental arrangement broadly available, also for small laboratories. The approach was successfully applied for the characterization of low molecular weight compounds separated on TLC plates, such as a mixture of pyrazole derivatives, alkaloids (nicotine and sparteine), and an extract from a drug tablet consisting of paracetamol, propyphenazone, and caffeine. The laser pointer used was capable of ablating organic compounds without the need of application of any additional substances (matrices, staining, etc.) on the TLC spots. The detection limit of the proposed method was estimated to be 35 ng/cm2 of a pyrazole derivative.
