Szczegóły publikacji
Opis bibliograficzny
Influence of polar and nonpolar carotenoids on structural and adhesive properties of model membranes / Dominika AUGUSTYŃSKA, Małgorzata Jemioła-Rzemińska, Kvetoslava BURDA, Kazimierz Strzałka // Chemico-Biological Interactions ; ISSN 0009-2797. — 2015 — vol. 239, s. 19–25. — Bibliogr. s. 24–25, Abstr. — Publikacja dostępna online od: 2015-06-20
Autorzy (4)
- AGHAugustyńska Dominika
- Jemioła-Rzemińska Małgorzata
- AGHBurda Kvetoslava
- Strzałka Kazimierz
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 95445 |
|---|---|
| Data dodania do BaDAP | 2016-02-10 |
| DOI | 10.1016/j.cbi.2015.06.021 |
| Rok publikacji | 2015 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Chemico-Biological Interactions |
Abstract
Carotenoids, which are known primarily for their photoprotective and antioxidant properties, may also strongly influence the physical properties of membranes. The localization and orientation of these pigments in the lipid bilayer depends on their structure and is determined by their interactions with lipid molecules. This affects both phase behavior and the mechanical properties of membranes. Differential scanning calorimetry (DSC) and atomic force microscopy (AFM) allowed us to gain a direct insight into the differences between the interaction of the non-polar beta-carotene and polar zeaxanthin embedded into DPPC liposomes. DSC results showed that zeaxanthin, having polar ionone rings, interacts more strongly with the membrane lipids than beta-carotene. The decrease in molar heat capacity by a factor of 2 with a simultaneous broadening of the main phase transition (gel-to-liquid crystalline phase transition) as compared to the two other systems studied suggests some increased length of the coupled interactions between the polar xanthophyll and lipids. Long-distance interactions lead to the formation of larger clusters which may exhibit higher flexibility than small clusters when only short-distance interactions occur. AFM experiments show that adhesive forces are 2 and 10 times higher for DPPC membranes enriched in beta-carotene and zeaxanthin, respectively, than those observed for an untreated system. Temperature dependent measurements of adhesion revealed that subphases can be formed in the gel lamellar state of DPPC bilayers. The presence of the non-polar carotenoid enhanced the effect and even a bifurcation of the substates was detected within a temperature range of 30.0-32.5 degrees C prior to pretransition. It is the first time when the presence of subphases has been demonstrated. This knowledge can be helpful in better understanding the functioning of carotenoids in biological membranes. AFM seem to be a very unique and sensitive method for detecting such fine changes in the lipid bilayers. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
