Szczegóły publikacji
Opis bibliograficzny
Oxygen plasma surface modification augments poly(L-lactide-co-glycolide) cytocompatibility toward osteoblasts and minimizes immune activation of macrophages / Anna Ścisłowska-Czarnecka, Dariusz Szmigiel, Michel Genet, Christine Dupont-Gillain, Elżbieta PAMUŁA, Elżbieta Kołaczkowska // Journal of Biomedical Materials Research. Part A ; ISSN 1549-3296. — 2015 — vol. 103 iss. 12, s. 3965–3977. — Bibliogr. s. 3977, Abstr. — Publikacja dostępna online od: 2015-08-30
Autorzy (6)
- Ścisłowska-Czarnecka Anna
- Szmigiel Dariusz
- Genet Michel J.
- Dupont-Gillain Christine C.
- AGHPamuła Elżbieta
- Kołaczkowska Elżbieta
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 95228 |
|---|---|
| Data dodania do BaDAP | 2016-01-28 |
| Tekst źródłowy | URL |
| DOI | 10.1002/jbm.a.35509 |
| Rok publikacji | 2015 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Journal of Biomedical Materials Research, Part A |
Abstract
Here, we report on modification of one of the model biomedical polymers, poly l-lactide-co-glycolide (PLGA; 85:15), by reactive ion etching (RIE) oxygen plasma treatment. PLGA's major disadvantage is high hydrophobicity which restrains binding of cell-adhesive proteins and host cells. In the current approach, we aimed to answer two questions: (1) will only short (10 s) and moderate (20-200 mTorr, 45-90 W) RIE oxygen plasma treatment, leading to decrease of water contact angle by only up to 10 degrees, sufficiently improve PLGA adherence to cells, and (2) how will this affect osteoblasts and activation of the immune system? All obtained modified PLGAs had improved hydrophilicity but unaltered roughness (as revealed by water contact angle measurements, X-ray photoelectron spectroscopy, and atomic force microscopy) resulting in significantly improved adhesion of osteoblasts (MG-63) and their low activation. Importantly, macrophages (RAW 264.7), one of the key cells initiating inflammation and bone resorption, responded significantly less vigorously to the modified polymers, expressing/releasing lower amounts of nitric oxide, matrix metalloproteinases (MMP-9), and pro-inflammatory cytokines (TNF-, IL-6, IL-12p70, IFN-, IL-10). We conclude that already slight RIE oxygen plasma modification of PLGA is sufficient to improve its surface properties, and enhance cytocompatibility. Most importantly, this type of modification prevents excessive immune response.