Szczegóły publikacji
Opis bibliograficzny
Influence of different types of carbon nanotubes on muscle cell response / Aneta FRĄCZEK-SZCZYPTA, Elżbieta Menaszek, Stanisław BŁAŻEWICZ, Jimi Adu, Ross Shevchenko, Tahmina Bahar Syeda, Anil Misra, Mohammad Alavijeh // Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems ; ISSN 0928-4931. — 2015 — vol. 46, s. 218–225. — Bibliogr. s. 225, Abstr.
Autorzy (8)
- AGHFrączek-Szczypta Aneta
- Menaszek Elżbieta
- AGHBłażewicz Stanisław
- Adu Jimi
- Shevchenko Ross
- Syeda Tahmina Bahar
- Misra Anil
- Alavijeh Mohammad S.
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 87257 |
|---|---|
| Data dodania do BaDAP | 2015-02-13 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.msec.2014.10.036 |
| Rok publikacji | 2015 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Materials Science and Engineering, C, Materials for Biological Applications |
Abstract
The aim of this study was to evaluate the impact of multi-walled carbon nanotubes (MWCNTs), before and after chemical surface functionalization on muscle cell response in vitro and in vivo conditions. Prior to biological tests the surface physicochemical properties of the carbon nanotubes (CNTs) deposited on a polymer membrane were investigated. To 'evaluate microstructure and structure of CNTs scanning electron microscopy (SEM) and Fourier transformation infrared spectroscopy (FTIR) were used. During in vitro study CNTs deposited on polymer membrane were contacted directly with myoblast cells, and after 7 days of culture cytotoxicity of samples was analyzed. Moreover, cell morphology in contact with CNTs was observed using SEM and fluorescence microscopy. The cytotoxicity of CNTs modified in a different way was comparable and significantly lower in comparison with pure polymer membrane. Microscopy analysis of cultured myoblasts confirms intense cell proliferation of all investigated samples with CNTs while for two kinds of CNTs myoblasts' differentiation into myotubes was observed. Histochemical reactions for the activity of enzymes such as acid phosphatase, cytochrome C oxidase, and non-specific esterase allowed the analysis of the extent of inflammation, degree of regeneration process of the muscle fibers resulting from the presence of the satellite cells and the neuromuscular junction on muscle fibers in contact with CNTs after implantation of CNTs into gluteal muscle of rat. © 2014 Elsevier B.V. All rights reserved.
