Szczegóły publikacji
Opis bibliograficzny
Temperature-responsive peptide-mimetic coating based on poly(N-methacryloyl-L-leucine) : properties, protein adsorption and cell growth / Joanna Raczkowska, [et al.], Andrzej BERNASIK, [et al.] // Colloids and Surfaces. B, Biointerfaces ; ISSN 0927-7765. — 2014 — vol. 118, s. 270–279. — Bibliogr. s. 278–279, Abstr.
Autorzy (14)
- Raczkowska Joanna
- AGHBernasik Andrzej
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 87139 |
|---|---|
| Data dodania do BaDAP | 2015-02-13 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.colurfb.2014.03.049 |
| Rok publikacji | 2014 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Colloids and Surfaces, B, Biointerfaces |
Abstract
Poly(N-methacryloyl-L-leucine) (PNML) coatings were successfully fabricated via polymerization from peroxide initiator grafted to premodified glass substrate. Chemical composition and thickness of PNML coatings were determined using time of flight-secondary ion mass spectrometry (TOF- SIMS) and ellipsometry, respectively. PNML coatings exhibit thermal response of the wettability, between 4 and 28 degrees C, which indicates a transition between hydrated loose coils and hydrophobic collapsed chains. Morphology of the PNML coating was observed with the AFM, transforming with increasing temperature from initially relatively smooth surface to rough and more structured surface. Protein adsorption observed by fluorescence microscopy for model proteins (bovine serum albumin and lentil lectin labeled with fluorescein isothiocyanate) at transition from 5 to 25 degrees C, showed high affinity of PNML coating to proteins at all investigated temperatures and pH. Thus, PNML coating have significant potential for medical and biotechnological applications as protein capture agents or functional replacements of antibodies ("plastic antibodies"). The high proliferation growth of the human embryonic kidney cell (HEM 293) onto PNML coating was demonstrated, indicating its excellent cytocompatibility. (C) 2014 Elsevier B.V. All rights reserved.