Szczegóły publikacji
Opis bibliograficzny
Removal of heavy metals from solution using biocompatible polymers / S. Govender, W. PRZYBYŁOWICZ, P. Swart // Desalination and Water Treatment ; ISSN 1944-3994 . — 2009 — vol. 9 iss. 1–3, s. 272–278. — Bibliogr. s. 278, Abstr. — The conference on membranes in drinking and industrial water production : 20–24 October 2008 : Toulouse, France
Autorzy (3)
- Govender Sagren
- AGHPrzybyłowicz Wojciech Józef
- Swart P.
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 49896 |
|---|---|
| Data dodania do BaDAP | 2010-01-27 |
| Rok publikacji | 2009 |
| Typ publikacji | referat w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Desalination and Water Treatment |
Abstract
Well characterised, hydrophobic, poly(vinylidene fluoride) and poly(sulfone) membranes, a physisorbed amphiphillic surfactant as an affinity linker with covalently attached bio-specific ligands to demonstrate heavy metal ion binding and re-use is reported. Central to this technology are the easy to manufacture membranes and a biocompatible Pluronic (TM) surfactant that serves multiple functions, where it acts not only as an affinity linker, preventing non-specific protein adsorption to the membrane surface but also non-covalently hydrophilises the membrane matrix. This work describes the fabrication and characterisation of non-porous, hydrophobic membranes for the attachment of a robust, bio-compatible ligand, in order to specifically chelate divalent cations (Cd(2+), Ni(2+), Zn(2+), Cu(2+)) and subsequently, histidine tagged proteins. These affinity tags are frequently used by bioengineers and biochemists to produce recombinant enzymes, peptides, hormones and antibodies. Successful coupling of ligands to Pluronic was achieved followed by quantification of the ligand binding sites on the surface. This technology is scalable (due to the multi-variant membrane module design), able to resist non-specific protein adsorption and can be regenerated and re-used (up to five times) with a simple anionic surfactant solution.
