Szczegóły publikacji
Opis bibliograficzny
Biological effects of polymers modified with carbon nanotubes on human osteoblast-like MG 63 cells / Lubica Grausova, Lucie Bacakova, Aneta FRĄCZEK, Stanisław BŁAŻEWICZ, Marta BŁAŻEWICZ, Michael Pepka // Inżynieria Biomateriałów = Engineering of Biomaterials / Polskie Stowarzyszenie Biomateriałów ; ISSN 1429-7248. — 2006 — R. 9 nr 58–60, s. 14–16. — Bibliogr. s. 16, Abstr.
Autorzy (6)
- Grausova Lubica
- Bacakova Lucie
- AGHFrączek-Szczypta Aneta
- AGHBłażewicz Stanisław
- AGHBłażewicz Marta
- Pepka Michael
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 30906 |
|---|---|
| Data dodania do BaDAP | 2007-01-11 |
| Tekst źródłowy | URL |
| Rok publikacji | 2006 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Inżynieria Biomateriałów = Engineering of Biomaterials |
Abstract
The tested materials were represented by a pure terpolymer of polytetrafluorethylene-polyvinyldifluoride-polypropylene (PTFE-PVDF-PP), pure polysulphone (PSU) and PSU modified with single- or multiwalled carbon nanotubes in concentrations of 0.5, 1 or 2 wt%. As control samples, a polystyrene cell culture dish and microscopic glass coverslips were used. The number and viability of human osteoblast-like MG 63 cells in cultures on these materials was detected with a Cell Viability Analyzer (Vi-CELL XR, Beckman Coulter) on 1, 3 and 7 days after seeding. On all tested samples, the cell number was similar or lower than that detected on the control polystyrene dishes. The cell viability on day 1 after seeding was relatively low on PTFE-PVDF-PP and some nanotube-containing samples, ranging from 10 to 100% of living cells, but on day 7 after seeding, it reached at least 90% on all tested samples. The cell spreading area was detected in cells after immunocytochemical staining of beta-actin on day 3 after seeding. In nanotube-containing samples, especially those with multi-walled nanotubes, this area was similar or even larger than that on the control materials. The beta-actin cytoskeleton was well developed in cells on all nanotube-containing materials and similar to that in cells on control surfaces. Thus, it can be concluded that nanotube-containing PSU supports the adhesion and growth of osteoblast-like cells and could be used for construction of bone implants in which the anchorage in the surrounding bone tissue is desirable.
