Szczegóły publikacji
Opis bibliograficzny
Nanoscale organization of adsorbed collagen: influence of substrate hydrophobicity and adsorption time / Elżbieta PAMUŁA, Vinciane De Cupere, Yves F. Dufrêne, Paul G. Rouxhet // Journal of Colloid and Interface Science ; ISSN 0021-9797. — 2004 — vol. 271 iss. 1, s. 80–91. — Bibliogr. s. 91, Abstr.
Autorzy (4)
- AGHPamuła Elżbieta
- de Cupere Vinciane
- Dufrêne Yves F.
- Rouxhet Paul G.
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 21621 |
|---|---|
| Data dodania do BaDAP | 2005-04-18 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.jcis.2003.11.012 |
| Rok publikacji | 2004 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Journal of Colloid and Interface Science |
Abstract
The adsorption of collagen on polystyrene (PS) and polystyrene oxidized by oxygen plasma discharge (PSox) was studied as a function of time using radiolabeling, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). Radiolabeling and XPS indicated that the initial step of adsorption was faster on PS than on PSox. AFM imaging under water revealed very different supramolecular organization of the adsorbed films depending on time and on the nature of the substrate: PS showed patterns of collagen aggregates at all adsorption times (from 1 min to 24 h); PSox was covered with a smooth layer except at long adsorption times (24 h), for which a mesh of collagen structures was observed. After fast drying, the collagen layer remained continuous and showed a morphology which recalled that observed under water. The mechanical stability of the adsorbed films was assessed under water by scraping with the AFM probe at different loading forces: no perturbations were created on PSox; in contrast, the layer adsorbed on PS was sensitive to scraping, the minimum force required to alter the collagen layer morphology increasing with time. These differences in the film properties were correlated with force measurements upon retraction: multiple adhesion forces were observed with collagen adsorbed on PS samples, whereas such an effect was never observed on PSox. The results show that the amount adsorbed and the organization of the adsorbed film respond differently to the adsorption time and that this is influenced by surface hydrophobicity. The quick initial adsorption on PS, compared to Box, is thought to leave dangling collagen segments that are responsible for the observed morphology, for adhesion forces, and for lower mechanical resistance of the adsorbed layer. (C) 2003 Elsevier Inc. All rights reserved.