Szczegóły publikacji
Opis bibliograficzny
Functionalization of metal-enriched Pleurotus biomass with $\gamma$-poly-glutamic acid for cytoprotective and antimicrobial wound care / Agata KRAKOWSKA, Alicja MATYJEWICZ, Iwona Skiba-Kurek, Rafał TWARÓG, Mateusz MARZEC, Zaneta Binert-Kusztal, Paulina Koczurkiewicz-Adamczyk, Bożena Muszyńska, Tomasz Skalski // Pharmaceuticals [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 1424-8247 . — 2026 — vol. 19 iss. 10 art. no. 1627, s. 1–31. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 27–31, Abstr. — Publikacja dostępna online od: 2026-10-14. — A. Krakowska – dod. afiliacja: Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland
Autorzy (9)
- AGHKrakowska Agata
- AGHMatyjewicz Alicja
- Skiba-Kurek Iwona
- AGHTwaróg Rafał
- AGHMarzec Mateusz M.
- Binert-Kusztal Żaneta
- Koczurkiewicz-Adamczyk Paulina
- Muszyńska Bożena
- Skalski Tomasz
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 171291 |
|---|---|
| Data dodania do BaDAP | 2026-11-10 |
| Tekst źródłowy | URL |
| DOI | 10.3390/ph19101627 |
| Rok publikacji | 2026 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Pharmaceuticals |
Abstract
Objectives: Chronic wound dressings require active components that support moisture management, biocompatibility, and local bioburden control. Therefore, in this study, freeze-dried biomass of Pleurotus ostreatus (Po) and Pleurotus djamor (Pdj) was cultivated in vitro under mineral-enriched conditions (calcium, magnesium, zinc) and subsequently modified with poly(gamma-glutamic acid) (gamma-PGA) to develop a bioactive fungal matrix intended as a functional additive for future wound-care biomaterials. Methods: The elemental content of the biomass was determined using atomic absorption spectroscopy (AAS), and the resulting biomaterial underwent physicochemical analyses-including scanning electron microscopy, X-ray diffraction (XRD), wettability measurements, differential scanning calorimetry (DSC), and thermogravimetric analysis (TG)-as well as biological performance assessments using a disk diffusion assay and cytotoxicity analysis. Results: Mineral enrichment enhanced Pleurotus spp. biomass production and metal accumulation, while gamma-PGA coating altered surface properties and cytocompatibility. Zinc supplementation significantly promoted biomass formation in both Pleurotus species, while metal accumulation depended on the specific element and fortification regime. gamma-PGA modification reduced the accessible BET surface area and formed a denser polymer-associated layer, confirmed by X-ray photoelectron spectroscopy. While the unmodified Po biomass exhibited the strongest in vitro antibacterial activity against S. aureus and P. aeruginosa, it also displayed dose-dependent cytotoxicity toward HaCaT keratinocytes at high concentrations. The addition of gamma-PGA moderately reduced the in vitro bacterial inhibition zones but provided a critical cytoprotective effect, mitigating the cytotoxicity of the concentrated fungal extracts and rescuing HaCaT-cell viability to meet ISO 10993-5 safety thresholds. Conclusions: These findings demonstrate that gamma-PGA-modified, metal-enriched Pleurotus biomass exhibits a distinct in vitro trade-off between absolute antimicrobial potency and cellular safety, supporting its further investigation as a biocompatible ingredient for multifunctional wound-dressing systems.
