Szczegóły publikacji
Opis bibliograficzny
Next-generation diabetes diagnostics: spectrochemical staging of red blood cells using vibrational spectroscopy / Aleksandra WILK, Natalia WILKOSZ, Marzena RUGIEŁ, Marta SALUGA, Kamil KAWOŃ, Tetiana STEPANENKO, Katarzyna BUŁAT, Aleksandra Wajda, Michał SZUWARZYŃSKI, Anna Zajączkowska, Anna Morgunowicz, Grzegorz Chodaczek, Víctor Navarro-Esteve, David Perez-Guaita, Joanna CHWIEJ, Katarzyna M. MARZEC // Journal of Advanced Research ; ISSN 2090-1232 . — 2026 — vol. 88, s. 415–436. — Bibliogr. s. 434–436, Abstr. — Publikacja dostępna online od: 2026-01-19. — T. Stepanenko – dod. afiliacja: Jagiellonian University, Kraków, Poland. — K. M. Marzec – dod. afiliacje: Jagiellonian University Medical College, Kraków, Poland ; VSB–Technical University of Ostrava, Czech Republic
Autorzy (16)
- AGHWilk Aleksandra
- AGHWilkosz Natalia
- AGHRugieł Marzena
- AGHSaługa Marta
- AGHKawoń Kamil
- AGHStepanenko Tetiana
- AGHBułat Katarzyna
- Wajda Aleksandra
- AGHSzuwarzyński Michał
- Zajączkowska Anna
- Morgunowicz Anna
- Chodaczek Grzegorz
- Navarro-Esteve Víctor
- Perez-Guaita David
- AGHChwiej Joanna
- AGHMarzec Katarzyna M.
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 170632 |
|---|---|
| Data dodania do BaDAP | 2026-10-19 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.jare.2026.01.052 |
| Rok publikacji | 2026 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Journal of Advanced Research |
Abstract
Introduction: Type 2 diabetes mellitus (T2DM) induces progressive biochemical and morphological alterations in red blood cells (RBCs), driven by hyperglycemia-induced oxidative stress and disrupted lipid-protein homeostasis. These changes compromise membrane integrity and serve as sensitive molecular indicators of disease severity. Yet, a non-invasive strategy for capturing such alterations at the molecular level remains an unresolved challenge. Objectives: This study employs Fourier Transform Infrared Spectroscopy (FT-IR) and Raman Spectroscopy (RS) to uncover sex-and age-specific molecular alterations in RBCs induced by T2DM. By integrating classical and advanced analytical techniques, we identify novel spectrochemical markers of disease progression, offering a non-invasive, label-free framework for staging and monitoring diabetic pathology at the molecular level. Methods: A total of 120 db/db and C57BL/6J mice were tested at 7, 12 and 24 weeks of age. Standard hematological and biochemical analyses were combined with vibrational spectroscopy (FT-IR, RS) and atomic force microscopy (AFM), to assess RBC morphology and molecular composition. Spectral data was analyzed using univariate and multivariate statistics to identify disease-specific spectral markers. Results: Partial Least Squares Discriminant Analysis reliably distinguished diabetic and control RBC profiles, confirming the presence of robust, disease-specific molecular patterns. Integration with hematological and biochemical parameters further validated the diagnostic relevance of this label-free, noninvasive approach. Structural protein analysis revealed a consistent decline in of alpha-helical content and an increase in beta-sheet and beta-turn structures, reflecting protein misfolding and aggregation, particularly in older diabetic females. Alterations in disulfide bonding, hydrated beta-sheets and H-bonded antiparallel beta-sheets highlighted oxidative stress-mediated membrane destabilization. Additionally, elevated plasma triglyceride levels and increased saturation of RBC membrane lipids indicate impaired lipid handling. Conclusion: Vibrational spectroscopy enables non-invasive, molecular-level profiling of diabetes-induced RBC alterations, revealing sex-and age-dependent signatures of disease progression. This approach offers a promising platform for precise staging and stratification in metabolic disorders.
