Szczegóły publikacji
Opis bibliograficzny
Effects of microgravity, hypergravity, and ionizing radiation on the enzymatic activity of proteinase K / Bartosz Rybacki, Wojciech Wysocki, Tomasz ZAJKOWSKI, Robert Brodzik, Beata Krawczyk // Molecules [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 1420-3049 . — 2026 — vol. 31 iss. 2 art. no. 229, s. 1–16. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 15–16, Abstr. — Publikacja dostępna online od: 2026-01-09. — T. Zajkowski - dod. afiliacja: Polish Astrobiology Society, Warsaw, Poland; Blue Marble Space Institute of Science, Seattle, USA
Autorzy (5)
- Rybacki Bartosz
- Wysocki Wojciech
- AGHZajkowski Tomasz
- Brodzik Robert
- Krawczyk Beata
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 165866 |
|---|---|
| Data dodania do BaDAP | 2026-02-23 |
| Tekst źródłowy | URL |
| DOI | 10.3390/molecules31020229 |
| Rok publikacji | 2026 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Molecules |
Abstract
Space conditions offer new insights into fundamental biological and molecular mechanisms. The study aimed to evaluate the enzymatic activity of proteinase K (PK) under extreme conditions relevant to space environments: simulated microgravity, hypergravity, and gamma radiation. PK activity was tested using azocasein (AZO) as a chromogenic substrate, with enzymatic reactions monitored spectrophotometrically at 450 nm. A rotating wall vessel (RWV) simulated microgravity, centrifugation at 1000× g (3303 rpm) generated hypergravity, and gamma radiation exposure used cesium-137 as the ionizing source. PK activity showed no remarkable changes under microgravity after 16 or 48 h; however, higher absorbance values after 96 h indicated enhanced AZO proteolysis compared to 1 g (Earth gravity) controls. In hypergravity, low PK concentrations exhibited slightly increased activity, while higher concentrations led to reduced activity. Meanwhile, gamma radiation caused a dose-dependent decline in PK activity; samples exposed to deep-space equivalent doses showed reduced substrate degradation. PK retained enzymatic activity under all tested conditions, though the type and duration of stress modulated its efficiency. The results suggest that enzyme-based systems may remain functional during space missions and, in some cases, exhibit enhanced activity. Nevertheless, their behavior must be evaluated in a context-dependent manner. These findings may be significant to advance biotechnology, diagnostics, and the development of enzyme systems for space applications.
