Szczegóły publikacji

Opis bibliograficzny

Carbyne-enriched carbon coatings on silicon chips as biosensing surfaces with stable-over-time biomolecule binding capacity / Dimitra Tsounidi, Panagiota Petrou, Mariya Aleksandrova, Tsvetozar Tsanev, Angeliki Tserepi, Evangelos Gogolides, Andrzej BERNASIK, Kamil Awsiuk, Natalia Janiszewska, Andrzej Budkowski, Ioannis Raptis // Nanomaterials [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN  2079-4991 . — 2025 — vol. 15 iss. 18 art. no. 1384, s. 1–16. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 14–16, Abstr. — Publikacja dostępna online od: 2025-09-09

Autorzy (11)

  • Tsounidi Dimitra
  • Petrou Panagiota S.
  • Aleksandrova Mariya
  • Tsanev Tsvetozar
  • Tserepi Angeliki
  • Gogolides Evangelos
  • AGHBernasik Andrzej
  • Awsiuk Kamil
  • Janiszewska Natalia
  • Budkowski Andrzej
  • Raptis Ioannis

Słowa kluczowe

carbon coatingsC reactive proteinbiosensorsamine biotin derivativecarbyne enrichedWhite Light Reflectance Spectroscopy

Dane bibliometryczne

ID BaDAP165050
Data dodania do BaDAP2026-01-08
Tekst źródłowyURL
DOI10.3390/nano15181384
Rok publikacji2025
Typ publikacjiartykuł w czasopiśmie
Otwarty dostęptak
Creative Commons
Czasopismo/seriaNanomaterials

Abstract

Carbyne-containing materials offer significant potential for biosensor applications due to their unique chemical and mechanical properties. In this study, carbyne-enriched carbon coatings deposited on SiO2/Si chips using ion-assisted pulse-plasma deposition were evaluated for the first time as substrates for optical biosensing. At first, the carbyne-enriched coatings were characterized by X-ray photoelectron spectroscopy, Raman spectroscopy, Atomic Force Microscopy, and the sessile drop method to assess their composition, structure, and wettability. After that, chips with carbyne-enriched coatings were modified with biomolecules through physical absorption or covalent bonding, and the respective biomolecular interactions were monitored in real-time by White Light Reflectance Spectroscopy (WLRS). In both cases, SiO2/Si chips modified with an aminosilane were used as reference substrates. Physical adsorption was tested through immobilization of an antibody against C-reactive protein (CRP) to enable its immunochemical detection, whereas covalent bonding was tested through coupling of biotin and monitoring its reaction with streptavidin. It was found that the carbyne-enriched carbon-coated chips retained both their antibody adsorption capability and their covalent bonding ability for over 18 months, while the modified with aminosilane SiO2/Si chips lost 90% of their antibody adsorption capacity and covalent bonding ability after two months of storage. These findings highlight the strong potential of carbyne-enriched carbon-coated chips as robust biosensing substrates, with applications extending beyond WLRS.