Szczegóły publikacji
Opis bibliograficzny
Cell sheet engineering platforms integrating antibacterial and thermo-responsive functionalities: Cu-nanoparticle-loaded P4VP brushes for retinal cell sheet harvesting / Anna Cieślik, Yana Shymborska, Svitlana Tymetska, Yurij Stetsyshyn, Andrzej BERNASIK, Monika Brzychczy-Włoch, Kamil Drożdż, Konrad Szajna, Franciszek Krok, Andrzej Budkowski, Joanna Raczkowska // Chemical Engineering Journal ; ISSN 1385-8947 . — 2025 — vol. 513 art. no. 162985, s. 1–19. — Bibliogr. s. 16–19, Abstr. — Publikacja dostępna online od: 2025-04-24
Autorzy (11)
- Cieślik Anna
- Shymborska Yana
- Tymetska Svitlana
- Stetsyshyn Yurij
- AGHBernasik Andrzej
- Brzychczy-Włoch Monika
- Drożdż Kamil
- Szajna Konrad
- Krok Franciszek
- Budkowski Andrzej
- Raczkowska Joanna
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 165039 |
|---|---|
| Data dodania do BaDAP | 2026-01-08 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.cej.2025.162985 |
| Rok publikacji | 2025 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Chemical Engineering Journal |
Abstract
Integrating antibacterial functionality directly into platforms for cell sheet engineering (CSE) would offer proactive defense against bacterial contamination and improve the safety and efficacy of CSE in cell-based therapies. This study demonstrates the potential of poly(4-vinylpyridine) polymer brushes with embedded copper nanoparticles (P4VP&Cu) as innovative CSE platforms, using the adult retinal pigment epithelial cell line (ARPE-19). The unique properties of such coatings, which combine the thermo-responsiveness of the polymer brush with the strong antibiocidal activity of copper, were traced using an interdisciplinary approach to provide information on the physicochemical properties of coatings, and confirm their cytocompatibility, and antibacterial effect through the contact- and release-killing mechanisms as a function of temperature. In particular, differentiated ARPE-19 cells cultured on P4VP coatings demonstrated spontaneous thermo-triggered detachment of intact cell sheets, which preserved their biological activity. This effect was maintained on P4VP&Cu coatings, which exhibited superior antibacterial activity without compromising cell viability in addition to resistance to protein adsorption. These findings highlight the potential of P4VP&Cu coatings as next-generation CSE platforms, combining effective antimicrobial defense with precise thermo-responsive functionality.
