Szczegóły publikacji
Opis bibliograficzny
SERS- and SEIRA-based characterization and sensing of highly selective bradykinin B2 receptor antagonists / Edyta PRONIEWICZ, Adam Prahl // International Journal of Molecular Sciences [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 1422-0067. — 2025 — vol. 26 iss. 16 art. no. 8089, s. 1-19. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 16-19, Abstr. — Publikacja dostępna online od: 2025-08-21
Autorzy (2)
- AGHProniewicz Edyta
- Prahl Adam
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 162378 |
|---|---|
| Data dodania do BaDAP | 2025-09-15 |
| Tekst źródłowy | URL |
| DOI | 10.3390/ijms26168089 |
| Rok publikacji | 2025 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | International Journal of Molecular Sciences |
Abstract
One of the major challenges in diagnosing various diseases, including neurological and neurodegenerative disorders, as well as carcinogenesis, is detecting unlabeled neurotransmitters. Surface-enhanced Raman spectroscopy (SERS) and surface-enhanced infrared spectroscopy (SEIRA) are promising methods for neurotransmitter biosensing and bioimaging. These methods are unique in that they are non-destructive and can identify molecular fingerprints. In this study, these methods were used to detect the following potent bradykinin (BK) antagonists: [D-Arg0,Hyp3,Thi5,D-Tic7,Oic8]BK, [D-Arg0,Hyp3,Thi5,D-Phe7,Thi8]BK, [D-Arg0,Hyp3,Igl5,D-Phe(5F)7,Oic8]BK, and [D-Arg0,Hyp3,Igl5,D-Igl7,Oic8]BK. The peptides were immobilized on a sensor surface consisting of silver (AgNPs) and gold (AuNPs) nanoparticles. These sensors have uniform particle sizes and small size distributions. Thanks to fast synthesis, easy handling, and reproducible results, these sensors enable routine testing. The vibrational structure of these peptides could not be determined using classical vibrational methods (Raman and IR) or surface-enhanced methods (SERS and SEIRA). This work presents the results of that research. Additionally, the SEIRA spectrum for BK or its analogs has not yet been published. This study presents research using SERS and SEIRA that shows that AgNP and AuNP sensors can detect the peptides under investigation. SERS is a more selective method than SEIRA because it allows for the differentiation of peptides based on the enhancement of certain bands in the SERS spectra. Furthermore, each peptide uniquely interacts with AuNPs, whereas all peptides bind to AgNPs via the C-terminus in different orientations. Consequently, the AuNP sensor is more selective than the AgNP sensor. Some bands were selected as markers for the sensing of specific peptides.
