Szczegóły publikacji
Opis bibliograficzny
Isolation of Citrus lemon extracellular vesicles: development and process control using capillary electrophoresis / Aleksandra Steć, Martyna Chodkowska, Joanna Kasprzyk-Pochopień, Przemysław MIELCZAREK, Wojciech Piekoszewski, Bogdan Lewczuk, Agata Płoska, Leszek Kalinowski, Bartosz Wielgomas, Szymon Dziomba // Food Chemistry ; ISSN 0308-8146. — 2023 — vol. 424 art. no. 136333, s. 1-10. — Bibliogr. s. 9-10, Abstr. — Publikacja dostępna online od: 2023-05-12
Autorzy (10)
- Steć Aleksandra
- Chodkowska Martyna
- Kasprzyk-Pochopień Joanna
- AGHMielczarek Przemysław
- Piekoszewski Wojciech
- Lewczuk Bogdan
- Płoska Agata
- Kalinowski Leszek
- Wielgomas Bartosz
- Dziomba Szymon
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 146894 |
|---|---|
| Data dodania do BaDAP | 2023-05-24 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.foodchem.2023.136333 |
| Rok publikacji | 2023 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Food Chemistry |
Abstract
A new and scalable method for the isolation of extracellular vesicles (EV) from Citrus lemon juice samples was developed. The methodology included preliminary preconcentration of the sample using ultrafiltration (UF) followed by size-exclusion chromatography (SEC) purification and final preconcentration of the eluates. Transmission electron microscopy and proteomic analysis showed that isolates contained exosome-like vesicles, exocyst-positive organelle (EXPO), and microvesicles. The efficiency of certain isolation steps was evaluated with total protein content assay (bicinchoninic acid assay, BCA), nanoparticles tracking analysis (NTA), and capillary electrophoresis (CE). A good correlation between CE, BCA, and NTA results was shown. The application of CE enabled the detection of soluble contaminants, macromolecular aggregates, and vesicles’ heterogeneity. The fluorescent staining of encapsulated nucleic acids was proposed for the identity confirmation of EV detected in CE. The study demonstrates the CE as a comprehensive tool for monitoring of the EV isolation process.
