Szczegóły publikacji
Opis bibliograficzny
A combined X-ray fluorescence and infrared microspectroscopy study for new insights into elemental-biomolecular obesity-induced changes in rat brain structures / Magdalena SZCZERBOWSKA-BORUCHOWSKA, Kaja Piana, Artur D. Surowka, Mateusz CZYŻYCKI, Paweł WRÓBEL, Maciej Szymkowski, Agata Ziomber-Lisiak // Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy ; ISSN 1386-1425. — 2023 — vol. 293 art. no. 122478, s. 1–15. — Bibliogr. s. 13–15, Abstr. — Publikacja dostępna online od: 2023-02-10. — M. Czyżycki - dod. afiliacje: Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany ; International Atomic Energy Agency, Seibersdorf, Austria
Autorzy (7)
- AGHSzczerbowska-Boruchowska Magdalena
- AGHPiana Kaja
- Surowka Artur D.
- AGHCzyżycki Mateusz
- AGHWróbel Paweł
- Szymkowski Maciej
- Ziomber-Lisiak Agata
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 145399 |
|---|---|
| Data dodania do BaDAP | 2023-02-22 |
| DOI | 10.1016/j.saa.2023.122478 |
| Rok publikacji | 2023 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Spectrochimica Acta, Part A, Molecular and Biomolecular Spectroscopy |
Abstract
The objective of our research was to determine the brain changes at the molecular and elemental levels typical of early-stage obesity. Therefore a combined approach using Fourier transform infrared micro-spectroscopy (FTIR-MS) and synchrotron radiation induced X-ray fluorescence (SRXRF) was introduced to evaluate some brain macromolecular and elemental parameters in high-calorie diet (HCD)- induced obese rats (OB, n = 6) and in their lean counterparts (L, n = 6). A HCD was found to alter the lipid- and protein- related structure and elemental composition of the certain brain areas important for energy homeostasis. The increased lipid unsaturation in the frontal cortex and ventral tegmental area, the increased fatty acyl chain length in the lateral hypothalamus and substantia nigra as well as the decreased both protein α helix to protein β- sheet ratio and the percentage fraction of β-turns and β-sheets in the nucleus accumbens were revealed in the OB group reflecting obesity-related brain biomolecular aberrations. In addition, the certain brain elements including P, K and Ca were found to differentiate the lean and obese groups at the best extent. We can conclude that HCD-induced obesity triggers lipid- and protein- related structural changes as well as elemental redistribution within various brain structures important for energy homeostasis. In addition, an approach applying combined X-ray and infrared spectroscopy was shown to be a reliable tool for identifying elemental-biomolecular rat brain changes for better understanding the interplay between the chemical and structural processes involved in appetite control.