Szczegóły publikacji
Opis bibliograficzny
Electrophoretic determination of trimethylamine (TMA) in biological samples as a novel potential biomarker of cardiovascular diseases methodological approach / Marek Konop, Mateusz Rybka, Emilia Waraksa, Anna K. Laskowska, Artur Nowiński, Tomasz Grzywacz, Wojciech J. KARWOWSKI, Adrian Drapała, Ewa Maria Kłodzińska // International Journal of Environmental Research and Public Health [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 1660-4601. — 2021 — vol. 18 iss. 23 art. no. 12318, s. 1–11. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 9–11, Abstr. — Publikacja dostępna online od: 2021-11-23
Autorzy (9)
- Konop Marek
- Rybka Mateusz
- Waraksa Emilia
- Laskowska Anna K.
- Nowiński Artur
- Grzywacz Tomasz
- AGHKarwowski Wojciech
- Drapała Adrian
- Kłodzińska Ewa
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 138140 |
|---|---|
| Data dodania do BaDAP | 2021-12-08 |
| Tekst źródłowy | URL |
| DOI | 10.3390/ijerph182312318 |
| Rok publikacji | 2021 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | International Journal of Environmental Research and Public Health |
Abstract
In competitive athletes, the differential diagnosis between nonpathological changes in cardiac morphology associated with training (commonly referred to as “athlete’s heart”) and certain cardiac diseases with the potential for sudden death is an important and not uncommon clinical problem. The use of noninvasive, fast, and cheap analytical techniques can help in making diagnostic differentiation and planning subsequent clinical strategies. Recent studies have demonstrated the role of gut microbiota and their metabolites in the onset and the development of cardiovascular diseases. Trimethylamine (TMA), a gut bacteria metabolite consisting of carnitine and choline, has recently emerged as a potentially toxic molecule to the circulatory system. The present work aims to develop a simple and cost-effective capillary electrophoresis-based method for the determination of TMA in biological samples. Analytical characteristics of the proposed method were evaluated through the study of its linearity (R2 > 0.9950) and the limit of detection and quantification (LOD = 1.2 µg/mL; LOQ = 3.6 µg/mL). The method shows great potential in high-throughput screening applications for TMA analysis in biological samples as a novel potential biomarker of cardiovascular diseases. The proposed electrophoretic method for the determination of TMA in biological samples from patients with cardiac disease is now in progress. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
