Szczegóły publikacji
Opis bibliograficzny
Lipid droplets in mammalian eggs are utilized during embryonic diapause / Roberta Arena, Simona Bisogno, Łukasz Gąsior, Joanna Rudnicka, Laura Bernhardt, Thomas Haaf, Federica Zacchini, Michał Bochenek, Kinga Fic, Ewelina Bik, Małgorzata Barańska, Anna BODZOŃ-KUŁAKOWSKA, Piotr SUDER, Joanna Depciuch, Artur Gurgul, Zbigniew Polański, Grażyna E. Ptak // Proceedings of the National Academy of Sciences of the United States of America ; ISSN 0027-8424. — 2021 — vol. 118 art. no. e2018362118, s. 1–9. — Bibliogr. s. 9–10. — Publikacja dostępna online od: 2021-03-01. — E. Bik - afiliacja: Jagiellonian University
Autorzy (17)
- Arena Roberta
- Bisogno Simona
- Gąsior Łukasz
- Rudnicka Joanna
- Bernhardt Laura
- Haaf Thomas
- Zacchini Federica
- Bochenek Michał
- Fic Kinga
- Bik Ewelina
- Barańska Małgorzata
- AGHBodzoń-Kułakowska Anna
- AGHSuder Piotr
- Depciuch Joanna
- Gurgul Artur
- Polański Zbigniew
- Ptak Grażyna E.
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 132803 |
|---|---|
| Data dodania do BaDAP | 2021-03-04 |
| Tekst źródłowy | URL |
| DOI | 10.1073/pnas.2018362118 |
| Rok publikacji | 2021 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Proceedings of the National Academy of Sciences of the United States of America |
Abstract
Embryonic diapause (ED) is a temporary arrest of an embryo at the blastocyst stage when it waits for the uterine receptivity signal to implant. ED used by over 100 species may also occur in normally “nondiapausing” mammals when the uterine receptivity signal is blocked or delayed. A large number of lipid droplets (LDs) are stored throughout the preimplantation embryo development, but the amount of lipids varies greatly across different mammalian species. Yet, the role of LDs in the mammalian egg and embryo remains unknown. Here, using a mouse model, we provide evidence that LDs play a crucial role in maintaining ED. By mechanical removal of LDs from zygotes, we demonstrated that delipidated embryos are unable to survive during ED. LDs are not essential for normal prompt implantation, without ED. We further demonstrated that with the progression of ED, the amount of intracellular lipid reduces, and composition changes. This decrease in lipid is caused by a switch from carbohydrate metabolism to lipid catabolism in diapausing blastocysts, which also exhibit increased release of exosomes reflecting elevated embryonic signaling to the mother. We have also shown that presence of LDs in the oocytes of various mammals positively corelates with their species-specific length of diapause. Our results reveal the functional role of LDs in embryonic development. These results can help to develop diagnostic techniques and treatment of recurrent implantation failure and will likely ignite further studies in developmental biology and reproductive medicine fields.