Szczegóły publikacji
Opis bibliograficzny
Enhanced cells anchoring to electrospun hybrid scaffolds with PHBV and HA particles for bone tissue regeneration / Joanna E. KARBOWNICZEK, Łukasz KANIUK, Krzysztof BERNIAK, Adam GRUSZCZYŃSKI, Urszula STACHEWICZ // Frontiers in Bioengineering and Biotechnology [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 2296-4185. — 2021 — vol. 9 art. no. 632029, s. 1–13. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 12–13. — Publikacja dostępna online od: 2021-02-17
Autorzy (5)
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 132798 |
|---|---|
| Data dodania do BaDAP | 2021-03-03 |
| Tekst źródłowy | URL |
| DOI | 10.3389/fbioe.2021.632029 |
| Rok publikacji | 2021 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Frontiers in Bioengineering and Biotechnology |
Abstract
Hybrid materials combining organic and inorganic compounds used as scaffolds are highly beneficial in bone regeneration. In this study, we successfully produced by blend electrospinning poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) scaffolds enriched with hydroxyapatite (HA) particles to biomimic bone tissue for improved and faster regeneration processes. The morphology, fiber diameters, and composition of the scaffolds were investigated by scanning electron microscopy (SEM) techniques followed by focused ion beam (FIB) sectioning to verify HA particles integration with PHBV fibers. In vitro cell culture was performed for 7 days and followed with the cell proliferation test (CellTiter-Blue R Assay). Additionally, cell integration with the scaffold was visualized by confocal and SEM imaging. We developed a simple way of obtaining hybrid scaffolds by electrospinning PHBV solution with HA particles without any post-processing. The PHBV + HA scaffold enhanced cell proliferation and filopodia formation responsible for cell anchoring within the created 3D environment. The obtained results show the great potential in the development of hybrid scaffolds stimulating bone tissue regeneration.
