Szczegóły publikacji
Opis bibliograficzny
Tailoring cellular microenvironments using scaffolds based on magnetically-responsive polymer brushes / Weronika Górka-Kumik, Paula Garbacz, Dorota LACHOWICZ, Paweł Dąbczyński, Szczepan Zapotoczny, Michał SZUWARZYŃSKI // Journal of Materials Chemistry. B ; ISSN 2050-750X. — 2020 — vol. 8 iss. 44, s. 10172–10181. — Bibliogr. s. 10180–10181, Abstr. — Publikacja dostępna online od: 2020-10-21
Autorzy (6)
- Górka-Kumik Weronika
- Garbacz Paula
- AGHLachowicz Dorota
- Dąbczyński Paweł
- Zapotoczny Szczepan
- AGHSzuwarzyński Michał
Dane bibliometryczne
| ID BaDAP | 131316 |
|---|---|
| Data dodania do BaDAP | 2020-12-04 |
| Tekst źródłowy | URL |
| DOI | 10.1039/d0tb01853h |
| Rok publikacji | 2020 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Journal of Materials Chemistry, B |
Abstract
A variety of polymeric scaffolds with the ability to control cell detachment has been created for cell culture using stimuli-responsive polymers. However, the widely studied and commonly used thermo-responsive polymeric substrates always affect the properties of the cultured cells due to the temperature stimulus. Here, we present a different stimuli-responsive approach based on poly(3-acrylamidopropyl)trimethylammonium chloride) (poly(APTAC)) brushes with homogeneously embedded superparamagnetic iron oxide nanoparticles (SPIONs). Neuroblastoma cell detachment was triggered by an external magnetic field, enabling a non-invasive process of controlled transfer into a new place without additional mechanical scratching and chemical/biochemical compound treatment. Hybrid scaffolds obtained in simultaneous surface-initiated atom transfer radical polymerization (SI-ATRP) were characterized by atomic force microscopy (AFM) working in the magnetic mode, secondary ion mass spectrometry (SIMS), and X-ray photoelectron spectroscopy (XPS) to confirm the magnetic properties and chemical structure. Moreover, neuroblastoma cells were cultured and characterized before and after exposure to a neodymium magnet. Controlled cell transfer triggered by a magnetic field is presented here as well. © 2020 The Royal Society of Chemistry.
