Szczegóły publikacji
Opis bibliograficzny
The new face of nucleolin in human melanoma / Dorota Hoja-Łukowicz, Małgorzata Przybyło, Ewa Pocheć, Anna Drabik, Jerzy Silberring, Marcelina Kremser, Dirk Schadendorf, Piotr Laidler, Anna Lityńska // Cancer Immunology Immunotherapy ; ISSN 0340-7004. — 2009 — vol. 58 iss. 9, s. 1471–1480. — Bibliogr. s. 1479–1480, Abstr. — Publikacja dostępna online od: 2009-04-12. — A. Drabik - afiliacja: Institute of Medical Biochemistry, Medical College, Jagiellonian University; Neurobiochemistry Department, Faculty of Chemistry, Jagiellonian University. — Network for the identification and validation of antigens and biomarkers in cancer and their application in clinical tumour immunology (ENACT) : 28–29 May, 2008, Nottingham, UK
Autorzy (9)
- Hoja-Łukowicz Dorota
- Przybyło Małgorzata
- Pocheć Ewa
- Drabik Anna
- Silberring Jerzy
- Kremser Marcelina
- Schadendorf Dirk
- Laidler Piotr
- Lityńska Anna
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 128761 |
|---|---|
| Data dodania do BaDAP | 2020-05-29 |
| Tekst źródłowy | URL |
| DOI | 10.1007/s00262-009-0705-8 |
| Rok publikacji | 2009 |
| Typ publikacji | referat w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Cancer Immunology Immunotherapy |
Abstract
Nucleolin is multifunctional protein mainly present in nucleoli but also detected in cytoplasm and plasma membranes. Extranuclear nucleolin differs from the nuclear form by its glycosylation. Studies on expression of nucleolin in breast cancer suggest a possible association to the metastatic cascade. In the present study, Vicia villosa lectin (VVL) precipitation followed by subsequent polyacrylamide gel electrophoresis and mass spectrometry analysis demonstrates nucleolin as a VVL-positive glycoprotein expressed in melanoma. The presence of VVL-positive nucleolin in the melanoma cell membrane and cytoplasm was confirmed by confocal microscopy. Using bioinformatic peptide prediction programs, nucleolin was shown to contain multiple possible MHC class-I binding peptides in its sequence which makes nucleolin an interesting melanoma marker and target for immunodiagnostic and possibly therapeutic purposes.
