Szczegóły publikacji
Opis bibliograficzny
Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells / Iwona M. WOJAK-ĆWIK, Łucja RUMIAN, Małgorzata KROK-BORKOWICZ, [et al.], Elżbieta PAMUŁA // Materials Science and Engineering. C, Materials for Biological Applications ; ISSN 0928-4931. — 2019 — vol. 97, s. 12–22. — Bibliogr. s. 21–22, Abstr. — Publikacja dostępna online od: 2018-12-07
Autorzy (11)
- AGHWojak-Ćwik Iwona
- AGHRumian Łucja Dobromiła
- AGHKrok-Borkowicz Małgorzata
- Hess R.
- Bernhardt Ricardo
- Dobrzyński Piotr
- Möller St.
- Schnabelrauch M.
- Hintze V.
- Scharnweber Dieter
- AGHPamuła Elżbieta
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 120658 |
|---|---|
| Data dodania do BaDAP | 2019-03-22 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.msec.2018.12.012 |
| Rok publikacji | 2019 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Materials Science and Engineering, C, Materials for Biological Applications |
Abstract
The main objective of this study was to enhance the biological performance of resorbable polymeric scaffolds for bone tissue engineering. Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC). Moreover, a new cell seeding method assuring 90% seeding efficiency on the scaffolds was developed. Poly(L-lactide-co-glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya). The application of two porogens resulted in bimodal pore distribution within the PLGA scaffolds as shown by scanning electron microscopy and microcomputer tomography. Two types of pores with diameters 400-600 urn and 2-20 mu m were obtained. The scaffolds were successfully coated with a homogenous layer of aECM as shown by Sirius red and toluidine blue staining. In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution. However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II. As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration.