Szczegóły publikacji
Opis bibliograficzny
Biochemical changes indicate developmental stage in the hippocampal formation / Joanna G. CHWIEJ, Stanisław W. Ciesielka, Agnieszka K. SKOCZEŃ, Krzysztof J. Janeczko, Christophe Sandt, Karolina L. PŁANETA, Zuzanna K. Setkowicz // ACS Chemical Neuroscience [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 1948-7193. — 2019 — vol. 10 iss. 1, s. 628–635. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 634–635, Abstr. — Publikacja dostępna online od: 2018-10-30
Autorzy (7)
- AGHChwiej Joanna
- AGHCiesielka Stanisław W.
- AGHDróżdż Agnieszka
- Janeczko Krzysztof
- Sandt Christophe
- AGHOlbrich Karolina L.
- Setkowicz-Janeczko Zuzanna
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 120165 |
|---|---|
| Data dodania do BaDAP | 2019-03-06 |
| Tekst źródłowy | URL |
| DOI | 10.1021/acschemneuro.8b00471 |
| Rok publikacji | 2019 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | ACS Chemical Neuroscience |
Abstract
The literature showing how age of humans or animals influences the IR absorption spectra recorded in different brain regions is very poor. A very limited number of studies used FTIR microspectroscopy for analysis of the aging process, however there is lack of data concerning the biomolecular changes occurring in the course of postnatal development of the central nervous system. Therefore, in this paper the topographic and semiquantitative biochemical changes occurring within the rat hippocampus during postnatal development were examined. To achieve the goal of the study, three groups of normal male rats differing in age were investigated. These were 6, 30, and 60 day old animals, and the chosen ages correspond to the neonatal period, childhood, and early adulthood in humans, respectively. Already, preliminary topographic analysis identified a number of significant changes in the accumulation of biomolecules within the hippocampal formation occurring during brain development. Such observation was confirmed by further semiquantitative analysis of intensities of selected absorption bands or ratios of their intensities. The detailed examinations were done for four hippocampal cellular layers (multiform, molecular, pyramidal, and granular layers), and the results showed that the accumulation of most biomolecules, including both saturated and unsaturated lipids as well as compounds containing phosphate and carbonyl groups, was significantly higher in adulthood comparing to the neonatal period. What is more, the increases in their levels were observed mostly between 6th and 30th days of animals' life. The unsaturation level of lipids did not change during postnatal development, although the differences in unsaturated and saturated lipids contents were noticed between examined animal groups. Significant differences in relative secondary structure of proteins were found between young adult rats and animals in neonatal period for which the relative level of proteins with beta-type secondary structure was the highest.
