Szczegóły publikacji
Opis bibliograficzny
Composites of gellan gum hydrogel enzymatically mineralized with calcium-zinc phosphate for bone regeneration with antibacterial activity / Timothy E. L. Douglas, Magdalena Pilarz, [et al.], Elżbieta PAMUŁA // Journal of Tissue Engineering and Regenerative Medicine ; ISSN 1932-6254. — 2017 — vol. 11 iss. 5, s. 1610–1618. — Bibliogr. s. 1617–1618, Abstr. — Publikacja dostępna online od: 2015-07-15
Autorzy (12)
- Douglas Timothy E. L.
- AGHPilarz Magdalena
- Lopez Marco
- Brackman Gilles
- Schaubroeck David
- Balcaen Lieve
- Bliznuk Vitaliy
- Dubruel Peter
- Knabe-Ducheyne Christine
- Vanhaecke Frank
- Coenye Tom
- AGHPamuła Elżbieta
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 110195 |
|---|---|
| Data dodania do BaDAP | 2017-12-10 |
| Tekst źródłowy | URL |
| DOI | 10.1002/term.2062 |
| Rok publikacji | 2017 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Journal of Tissue Engineering and Regenerative Medicine |
Abstract
Gellan gum hydrogels functionalized with alkaline phosphatase were enzymatically mineralized with phosphates in mineralizationmedium containing calcium (Ca) and zinc (Zn) to improve their suitability as biomaterials for bone regeneration. The aims of the study were to endow mineralized hydrogels with antibacterial activity by incorporation of Zn in the inorganic phase, and to investigate the effect of Zn incorporation on the amount and type of mineral formed, the compressive modulus of the mineralized hydrogels and on their ability to support adhesion and growth of MC3T3-E1 osteoblast-like cells. Mineralization medium contained glycerophosphate (0.05 m) and three different molar Ca: Zn ratios, 0.05: 0, 0.04:0.01 and 0.025:0.025 (all mol/dm(3)), hereafter referred to as A, B and C, respectively. FTIR, SAED and TEM analysis revealed that incubation for 14 days caused the formation of predominantly amorphous mineral phases in sample groups A, B and C. The presence of Zn in sample groups B and C was associated with a drop in the amount of mineral formed and a smaller mineral deposit morphology, as observed by SEM. ICP-OES revealed that Zn was preferentially incorporated into mineral compared to Ca. Mechanical testing revealed a decrease in compressive modulus in sample group C. Sample groups B and C, but not A, showed antibacterial activity against biofilm-forming, methicillin-resistant Staphylococcus aureus. All sample groups supported cell growth. Zn incorporation increased the viable cell number. The highest values were seen on sample group C. In conclusion, the sample group containing the most Zn, i.e. group C, appears to be the most promising.
