Szczegóły publikacji
Opis bibliograficzny
Bioinspired, biomimetic, double-enzymatic mineralization of hydrogels for bone regeneration with calcium carbonate / Marco A. Lopez-Heredia, [et al.], Agata Łapa, [et al.], Elżbieta PAMUŁA, [et al.] // Materials Letters ; ISSN 0167-577X. — 2017 — vol. 190, s. 13–16. — Bibliogr. s. 16, Abstr. — Publikacja dostępna online od: 2016-12-31
Autorzy (15)
- Lopez-Heredia Marco A.
- AGHŁapa Agata
- Mendes Ana C.
- Balcaen Lieve
- Samal Sangram K.
- Chai Feng
- Voort Pascal van der
- Stevens Christian V.
- Parakhonskiy Bogdan V.
- Chronakis Ioannis S.
- Vanhaecke Frank
- Blanchemain Nicholas
- AGHPamuła Elżbieta
- Skirtach Andre G.
- Douglas Timothy E. L.
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 104578 |
|---|---|
| Data dodania do BaDAP | 2017-05-16 |
| Tekst źródłowy | URL |
| DOI | 10.1016/j.matlet.2016.12.122 |
| Rok publikacji | 2017 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Czasopismo/seria | Materials Letters |
Abstract
Hydrogels are popular materials for tissue regeneration. Incorporation of biologically active substances, e.g. enzymes, is straightforward. Hydrogel mineralization is desirable for bone regeneration. Here, hydrogels of Gellan Gum (GG), a biocompatible polysaccharide, were mineralized biomimetically with CaCO3 using a double enzymatic approach. The enzymes urease (U) and carbonic anhydrase (CA) were incorporated in GG hydrogels. Hydrogels were incubated in a mineralization solution containing U substrate (urea) and calcium ions. U converts urea to ammonia (which raises pH) and CO2. CA catalyses the reaction of CO2 with water to form HCO3−, which undergoes deprotonation to form CO32−, which react with Ca2+ to form insoluble CaCO3. All hydrogels containing U+CA were mineralized more with calcite and stiffer than hydrogels containing U. Mineralization with calcite promoted proliferation and spreading of osteoblast-like cells.
