Szczegóły publikacji
Opis bibliograficzny
Milk-derived carbon quantum dots: study of biological and chemical properties provides evidence of toxicity / Hasan SHABBIR, Konrad WOJTASZEK, Bogdan RUTKOWSKI, Edit Csapó, Marek Bednarski, Anita Adamiec, Monika Głuch-Lutwin, Barbara Mordyl, Julia Druciarek, Magdalena Kotańska, Piotr Ozga, Marek WOJNICKI // Molecules [Dokument elektroniczny]. — Czasopismo elektroniczne ; ISSN 1420-3049. — 2022 — vol. 27 iss. 24 art. no. 8728, s. 1–19. — Wymagania systemowe: Adobe Reader. — Bibliogr. s. 17–19, Abstr. — Publikacja dostępna online od: 2022-12-09
Autorzy (12)
- AGHShabbir Hasan
- AGHWojtaszek Konrad
- AGHRutkowski Bogdan
- Csapó Edit
- Bednarski Marek
- Adamiec Anita
- Głuch-Lutwin Monika
- Mordyl Barbara
- Druciarek Julia
- Kotańska Magdalena
- Ozga Piotr
- AGHWojnicki Marek
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 144158 |
|---|---|
| Data dodania do BaDAP | 2022-12-20 |
| Tekst źródłowy | URL |
| DOI | 10.3390/molecules27248728 |
| Rok publikacji | 2022 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Molecules |
Abstract
Carbon dots (CDs) are carbon-based zero-dimensional nanomaterials that can be prepared from a number of organic precursors. In this research, they are prepared using fat-free UHT cow milk through the hydrothermal method. FTIR analysis shows C=O and C-H bond presence, as well as nitrogen-based bond like C-N, C=N and –NH2 presence in CDs, while the absorption spectra show the absorption band at 280 ± 3 nm. Next, the Biuret test was performed, with the results showing no presence of unreacted proteins in CDs. It can be said that all proteins are converted in CDs. Photo luminance spectra shows the emission of CDs is 420 nm and a toxicity study of CDs was performed. The Presto Blue method was used to test the toxicity of CDs for murine hippocampal cells. CDs at a concentration of 4 mg/mL were hazardous independent of synthesis time, while the toxicity was higher for lower synthesis times of 1 and 2 h. When the concentration is reduced in 1 and 2 h synthesized CDs, the cytotoxic effect also decreases significantly, ensuring a survival rate of 60–80%. However, when the synthesis time of CDs is increased, the cytotoxic effect decreases to a lesser extent. The CDs with the highest synthesis time of 8 h do not show a cytotoxic effect above 60%. The cytotoxicity study shows that CDs may have a concentration and time–dependent cytotoxic effect, reducing the number of viable cells by 40%.