Szczegóły publikacji
Opis bibliograficzny
Efficient conjugation of anti-HBsAg antibody to modified core-shell magnetic nanoparticles $(Fe_{3}O_{4}@SiO_{2}/NH_{2})$ / Shahram Parvaneh, Fatemeh Khademi, Gisya ABDI, Abdolhamid Alizadeh, Ali Mostafaie // Bioimpacts ; ISSN 2228-5652. — 2021 — vol. 11 iss. 4, s. 237–244. — Bibliogr. s. 243–244, Abstr. — Publikacja dostępna online od: 2020-04-28. — A. Alizadeh - błędnie przypisany do AGH
Autorzy (5)
- Parvaneh Shahram
- Khademi Fatemeh
- AGHAbdi Gisya
- Alizadeh Abdolhamid
- Mostafaie Ali
Słowa kluczowe
Dane bibliometryczne
| ID BaDAP | 135612 |
|---|---|
| Data dodania do BaDAP | 2021-09-02 |
| Tekst źródłowy | URL |
| DOI | 10.34172/bi.2021.34 |
| Rok publikacji | 2021 |
| Typ publikacji | artykuł w czasopiśmie |
| Otwarty dostęp |  |
| Creative Commons | |
| Czasopismo/seria | Bioimpacts |
Abstract
Introduction: Further development of magnetic-based detection techniques could be of significant use in increasing the sensitivity of detection and quantification of hepatitis B virus (HBV) infection. The present work addresses the fabrication and characterization of a new bio-nano composite based on the immobilization of goat anti-HBsAg antibody on modified core-shell magnetic nanoparticles (NPs) by (3-aminopropyl) triethoxysilane (APTES), named Fe3O4@SiO2/NH2, and magnetic NPs modified by chitosan (Fe3O4@CS). Methods: At the first step, Fe3O4 was modified with the silica and APTES (Fe3O4@SiO2/NH2) and chitosan (Fe3O4@CS) separately. The goat anti-HBsAg antibody was activated by two different protocols: Sodium periodate and EDC-NHS. Then the resulted composites were conjugated with activated goat anti-HBsAg IgG. An external magnet collected Bio-super magnetic NPs (BSMNPs) and the remained solution was analyzed by the Bradford method to check the amount of attached antibody to the surface of BSMNPs. Results: The findings indicated that activation of antibodies by sodium periodate method 15-17 mu g antibody immobilized on 1 mg of super magnetic nanoparticles (SMNPs). However, in the EDC-NHS method, 8-10 mu g of antibody was conjugated with 1 mg of SMNPs. The resulting bio-magnetic NPs were applied for interaction with the HBsAg target using enzyme-linked immunosorbent assay (ELISA). About 1 mu g antigen attached to 1 mg SMNPs, which demonstrated that the fabricated materials are applicable in the detection scope of HBsAg. Conclusion: In the present study, we developed new antibody-conjugated magnetic NPs for the detection of HBsAg using an efficient conjugation strategy. The results demonstrated that the binding capacity of Fe3O4@SiO2/NH2 was comparable with commercially available products. Our designed method for conjugating anti-HBsAg antibody to a magnetic nanoparticle opens the way to produce a high capacity of magnetic NPs.